Laboratory of Molecular Immunopathology, Clinical Hospital, Federal University of Paraná, Curitiba, Paraná, Brazil.
Laboratory of Human Molecular Genetics, Department of Genetics, Federal University of Paraná, Curitiba, Paraná, Brazil.
Parasite Immunol. 2021 Jun;43(6):e12829. doi: 10.1111/pim.12829. Epub 2021 Mar 18.
To investigate whether FCN3 polymorphisms and circulating ficolin-3 levels were associated with clinical forms of chronic Chagas disease (CD) and to assess their potential use as biomarkers for the disease or its severity.
FCN3 polymorphisms (g.1637delC (rs532781899) in exon 5; g.3524_3532insTATTTGGCC (rs28362807) in intron 5 and g.4473C > A) (rs4494157) in intron 7) were determined in 178 chronic CD patients (65 asymptomatic, 68 cardiac, 21 digestive and 24 cardiodigestive), and 285 healthy controls by sequence-specific PCR. Ficolin-3 serum levels, measured by ELISA in 80 patients and 80 controls, did not differ between groups. On the other hand, ficolin-3 levels were positively correlated with left ventricular ejection fraction (P = .002; r = .5), with lower levels associated with increased risk of cardiac insufficiency (P = .033; OR 7.21, 95%IC 1.17-44.4). Ficolin-3 levels were positively correlated with ficolin-2 (P = .021; r = .63), and negatively with MBL (P = .002; r = -.36) and pentraxin-3 (P = .04; r = -.32) levels. No significant results were observed for the investigated FCN3 polymorphisms and CD. The g.1637del/1637C heterozygotes presented lower ficolin-3 levels than g.1637C/1637C homozygotes in the control group (P = .023).
Low ficolin-3 levels may play a role in the pathophysiology of cardiac insufficiency associated with CD.
研究 FCN3 多态性和循环 ficolin-3 水平是否与慢性恰加斯病（CD）的临床形式相关，并评估其作为疾病或其严重程度的生物标志物的潜在用途。
通过序列特异性 PCR ，在 178 例慢性 CD 患者（65 例无症状、68 例心脏、21 例消化和 24 例心脏消化）和 285 例健康对照中确定了 FCN3 多态性（外显子 5 中的 g.1637delC(rs532781899)；内含子 5 中的 g.3524_3532insTATTTGGCC(rs28362807)和内含子 7 中的 g.4473C> A(rs4494157))。通过 ELISA 在 80 例患者和 80 例对照中测量 ficolin-3 血清水平，两组之间无差异。另一方面，ficolin-3 水平与左心室射血分数呈正相关（P=0.002；r=0.5），水平较低与心脏功能不全的风险增加相关（P=0.033；OR 7.21，95%CI 1.17-44.4）。ficolin-3 水平与 ficolin-2 呈正相关（P=0.021；r=0.63），与 MBL（P=0.002；r=-.36）和 pentraxin-3（P=0.04；r=-.32）呈负相关。未观察到研究的 FCN3 多态性与 CD 之间存在显著结果。在对照组中，g.1637del/1637C 杂合子的 ficolin-3 水平低于 g.1637C/1637C 纯合子（P=0.023）。
低 ficolin-3 水平可能在与 CD 相关的心脏功能不全的病理生理学中发挥作用。
