Department of NanoEngineering, Chemical Engineering Program, Moores Cancer Center, University of California San Diego, La Jolla, California 92093, United States.
Nano Lett. 2021 Mar 24;21(6):2603-2609. doi: 10.1021/acs.nanolett.1c00238. Epub 2021 Mar 9.
Natural cell membranes derived from various cell sources have been successfully utilized to coat nanomaterials for functionalization. However, intracellular membranes from the organelles of eukaryotes remain unexplored. Herein, we choose mitochondrion as a representative cell organelle and coat outer mitochondrial membrane (OMM) from mouse livers onto nanoparticles and field-effect transistors (FETs) through a membrane vesicle-substrate fusion process. Polymeric nanoparticles coated with OMM (OMM-NPs) can bind with ABT-263, a B-cell lymphoma protein 2 (Bcl-2) inhibitor that targets the OMM. As a result, OMM-NPs effectively protect the cells from ABT-263 induced cell death and apoptosis and attenuated ABT-263-induced thrombocytopenia . Meanwhile, FET sensors coated with OMM (OMM-FETs) can detect and distinguish anti-Bcl-2 antibody and small molecule agonists. Overall, these results show that OMM can be coated onto the surfaces of both nanoparticles and functional devices, suggesting that intracellular membranes can be used as coating materials for novel biointerfacing.
天然细胞膜来源于各种细胞来源，已成功用于纳米材料的功能化涂层。然而，真核生物细胞器的细胞内膜仍未被探索。在此，我们选择线粒体作为代表性的细胞器，并通过膜囊泡-底物融合过程将来自小鼠肝脏的外线粒体膜 (OMM) 涂覆到纳米颗粒和场效应晶体管 (FET) 上。涂覆有 OMM 的聚合物纳米颗粒（OMM-NPs）可以与 ABT-263 结合，ABT-263 是一种针对 OMM 的 B 细胞淋巴瘤蛋白 2 (Bcl-2) 抑制剂。结果，OMM-NPs 有效保护细胞免受 ABT-263 诱导的细胞死亡和细胞凋亡，并减轻 ABT-263 诱导的血小板减少症。同时，涂覆有 OMM 的 FET 传感器（OMM-FETs）可以检测和区分抗 Bcl-2 抗体和小分子激动剂。总的来说，这些结果表明 OMM 可以涂覆在纳米颗粒和功能器件的表面上，这表明细胞内膜可以用作新型生物界面的涂层材料。
