Department of Medical Biotechnology and Laboratory Science, and Division of Biotechnology, Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
Department of Life Sciences, and Ph.D. Program in Translational Medicine, National Chung Hsing University, Taichung, Taiwan.
Methods Mol Biol. 2021;2269:83-92. doi: 10.1007/978-1-0716-1225-5_6.
A co-culture model of mesenchymal stem cells (MSCs) and fibroblasts is an efficient and rapid method to evaluate the anti-fibrotic effects of MSCs-based cell therapy. Transforming growth factor (TGF)-β1 plays a key role in promotion of fibroblast activation and differentiation which can induce collagen deposition, increase ECM production in lung tissue, eventually resulted in pulmonary fibrosis. Here, we use this co-culture system and examine the ECM production in activated fibroblasts by western blot and quantitative real-time analysis to understand the therapeutic effects of MSCs.
间充质干细胞 (MSCs) 和成纤维细胞的共培养模型是评估基于 MSC 的细胞疗法抗纤维化效果的有效且快速的方法。转化生长因子 (TGF)-β1 在促进成纤维细胞活化和分化中发挥关键作用，可诱导胶原沉积，增加肺组织中细胞外基质的产生，最终导致肺纤维化。在这里，我们使用这种共培养系统，并通过蛋白质印迹和实时定量分析来检测活化的成纤维细胞中细胞外基质的产生，以了解 MSC 的治疗效果。
