Interuniversity Research Institute for Molecular Recognition and Technological Development (IDM), Polytechnic University of Valencia, University of Valencia, Burjasot, Valencia, Spain.
Department of Pharmacy, Faculty of Health Sciences, Cardenal Herrera-CEU University, Alfara del Patriarca, Valencia, Spain.
Methods Mol Biol. 2021;2269:221-231. doi: 10.1007/978-1-0716-1225-5_15.
Adipose tissue-derived mesenchymal stem cells (AD-MSCs) offer great therapeutic potential for osteoarthritis (OA) treatment. Recent investigations have revealed the contribution of extracellular vesicles (EVs) to AD-MSC actions. Here, we describe a method to study the in vitro effects of EVs from AD-MSCs in OA chondrocytes. This chapter includes the isolation and analysis of human AD-MSCs and their EVs as well as the isolation and treatment of OA chondrocytes.
脂肪组织来源的间充质干细胞（AD-MSCs）为骨关节炎（OA）的治疗提供了巨大的治疗潜力。最近的研究揭示了细胞外囊泡（EVs）对 AD-MSC 作用的贡献。在这里，我们描述了一种研究 AD-MSC 来源的 EV 在 OA 软骨细胞中体外作用的方法。本章包括人 AD-MSCs 及其 EV 的分离和分析，以及 OA 软骨细胞的分离和处理。
