评估来源于脂肪组织间充质干细胞的细胞外囊泡对骨关节炎患者原代人软骨细胞的作用。
Evaluation of Extracellular Vesicles from Adipose Tissue-Derived Mesenchymal Stem Cells in Primary Human Chondrocytes from Patients with Osteoarthritis.
机构信息
Interuniversity Research Institute for Molecular Recognition and Technological Development (IDM), Polytechnic University of Valencia, University of Valencia, Burjasot, Valencia, Spain.
Department of Pharmacy, Faculty of Health Sciences, Cardenal Herrera-CEU University, Alfara del Patriarca, Valencia, Spain.
出版信息
Methods Mol Biol. 2021;2269:221-231. doi: 10.1007/978-1-0716-1225-5_15.
Adipose tissue-derived mesenchymal stem cells (AD-MSCs) offer great therapeutic potential for osteoarthritis (OA) treatment. Recent investigations have revealed the contribution of extracellular vesicles (EVs) to AD-MSC actions. Here, we describe a method to study the in vitro effects of EVs from AD-MSCs in OA chondrocytes. This chapter includes the isolation and analysis of human AD-MSCs and their EVs as well as the isolation and treatment of OA chondrocytes.
脂肪组织来源的间充质干细胞（AD-MSCs）为骨关节炎（OA）的治疗提供了巨大的治疗潜力。最近的研究揭示了细胞外囊泡（EVs）对 AD-MSC 作用的贡献。在这里，我们描述了一种研究 AD-MSC 来源的 EV 在 OA 软骨细胞中体外作用的方法。本章包括人 AD-MSCs 及其 EV 的分离和分析，以及 OA 软骨细胞的分离和处理。
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