Departamento de Microbiología y Parasitología, Facultad de Farmacia, Campus Sur 15782, Universidad de Santiago de Compostela, A Coruña, Spain.
Institute Maimónides of Biomedica Investigation of Córdoba, Córdoba, Spain.
Methods Mol Biol. 2021;2259:205-213. doi: 10.1007/978-1-0716-1178-4_13.
Classical and culture-based methods for the identification and characterization of the biochemical properties of microorganisms are slow and labor-intensive. Liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) has been used for the analysis of bacterial pathogen strain-specific diagnostic peptides allowing the characterization of bacterial strains.Here, we describe the analysis of tryptic digestion peptides by LC-ESI-MS/MS to search for specific biomarkers useful for the rapid identification of, on the one hand, the bacterial species and, on the other hand, the physiological and biochemical characteristics such as the expression of virulence factors, including toxins, immune-modulatory factors, and exoenzymes.
经典的和基于文化的方法用于鉴定和描述微生物的生化特性是缓慢和劳动密集型的。液相色谱-电喷雾电离-串联质谱（LC-ESI-MS/MS）已被用于分析细菌病原体菌株特异性诊断肽，从而能够对细菌菌株进行特征描述。在这里，我们描述了通过 LC-ESI-MS/MS 分析胰蛋白酶消化肽，以寻找用于快速鉴定细菌物种的特定生物标志物，一方面，另一方面，还可以鉴定生理和生化特征，如毒力因子的表达，包括毒素、免疫调节因子和外酶。
