一种快速细菌鉴定的简单鸟枪法蛋白质组学方法。
A simple shotgun proteomics method for rapid bacterial identification.
机构信息
National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.
出版信息
J Microbiol Methods. 2013 Jul;94(1):54-7. doi: 10.1016/j.mimet.2013.04.008. Epub 2013 Apr 28.
PMID:23631909
Abstract
Bacterial pathogens were rapidly identified by shotgun proteomics using a novel, easy to implement database search strategy. Peptide sequence data from nano-LC-MS/MS was searched against a database represented by concatenated proteomes of completed genome sequences. Select bacterial species, including BSL-3 select agents, were used to demonstrate this method.
摘要
通过使用新型、易于实施的数据库搜索策略，利用鸟枪法蛋白质组学技术可以快速鉴定细菌病原体。来自纳升液相色谱-串联质谱的肽序列数据与由已完成基因组序列的串联蛋白质组代表的数据库进行搜索。选择包括 BSL-3 选择剂在内的特定细菌物种来验证该方法。
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