Liu Chao, Zheng Yanfeng, Li Xia, Li Baixue, Wen Li, Wang Dong, Feng Quansheng, Jiang Cen
College of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.
Evid Based Complement Alternat Med. 2021 Feb 22;2021:6291428. doi: 10.1155/2021/6291428. eCollection 2021.
Although our previous studies revealed that H2BE exhibited significantly differential expression between two CHB TCM-syndromes: Spleen-stomach dampness-heat syndrome, SSDHS and liver-depression spleen-deficiency syndrome, LDSDS, the underlying mechanisms remain largely unknown. Recent studies showed that dynamic expression fluctuation of Th related cytokines in CHB TCM-syndromes, and furthermore, their expression levels were largely regulated by H2BE. This study aims to detect the expression level differences of Th related cytokines between these two TCM-syndromes and further investigate the underlying regulatory mechanisms. The expression levels of the four Th related cytokines and H2BE were analyzed and the protein-protein interaction networks between H2BE and the four cytokines were constructed. Our results suggested that almost all the cytokines were significantly upregulated compared with the healthy group ( < 0.05). Interestingly, among the four cytokines, only IL-4 and INF- showed statistical significance between these two syndromes. The protein-protein interaction networks demonstrated that H2BE was indirectly associated with IL-4 and IL-10, and H2BE may regulate the expression levels of cytokines through GATA3. Taken together, our results indicated that IL-4 and INF- are two representative cytokines that may serve as two potential biochemical indicators of SSDHS and LDSDS in CHB patients; except what has been reported, our study found that one possible way for H2BE to regulate the expression of cytokines is to interact with GATA3 directly or indirectly.
尽管我们之前的研究表明，H2BE在两种慢性乙型肝炎中医证型（脾胃湿热证和肝郁脾虚证）之间表现出显著差异表达，但其潜在机制仍 largely未知。最近的研究表明，慢性乙型肝炎中医证型中Th相关细胞因子存在动态表达波动，此外，它们的表达水平在很大程度上受H2BE调节。本研究旨在检测这两种中医证型之间Th相关细胞因子的表达水平差异，并进一步探究潜在的调控机制。分析了四种Th相关细胞因子和H2BE的表达水平，并构建了H2BE与这四种细胞因子之间的蛋白质-蛋白质相互作用网络。我们的结果表明，与健康组相比，几乎所有细胞因子均显著上调（<0.05）。有趣的是，在这四种细胞因子中，只有IL-4和INF-在这两种证型之间表现出统计学意义。蛋白质-蛋白质相互作用网络表明，H2BE与IL-4和IL-10间接相关，且H2BE可能通过GATA3调节细胞因子的表达水平。综上所述，我们的结果表明，IL-4和INF-是两种代表性细胞因子，可能作为慢性乙型肝炎患者脾胃湿热证和肝郁脾虚证的两种潜在生化指标；除已报道的内容外，我们的研究发现，H2BE调节细胞因子表达的一种可能方式是直接或间接与GATA3相互作用。
