铍化合物溶解度对犬体外肺泡巨噬细胞细胞毒性的影响。

The effect of beryllium compound solubility on in vitro canine alveolar macrophage cytotoxicity.

作者信息

Finch G L, Verburg R J, Mewhinney J A, Eidson A F, Hoover M D

机构信息

Lovelace Biomedical and Environmental Research Institute, Albuquerque, NM 87185-5890.

出版信息

Toxicol Lett. 1988 May;41(2):97-105. doi: 10.1016/0378-4274(88)90083-5.

DOI:10.1016/0378-4274(88)90083-5

PMID:3368931

Abstract

Pulmonary alveolar macrophage cells (PAM), obtained by bronchopulmonary lavage of Beagle dogs, were exposed in vitro to beryllium oxide (BeO) particles calcined at either 500 or 1000 degrees C or to beryllium sulfate (BeSO4). Cell viability was determined by trypan blue dye exclusion after 20 h in culture. The most toxic material tested was BeSO4, followed by BeO calcined at 500 degrees C, then BeO calcined at 1000 degrees C. An in vitro dissolution technique was used to measure the relative solubility of the BeO particles. The BeO prepared at 500 degrees C exhibited greater solubility compared with BeO prepared at 1000 degrees C. This study extends previous work by examining the effects of beryllium compounds on canine PAM, and by relating PAM cytotoxicity with measured values of beryllium compound solubility.

摘要

通过对比格犬进行支气管肺泡灌洗获得的肺泡巨噬细胞（PAM），在体外暴露于500或1000摄氏度煅烧的氧化铍（BeO）颗粒或硫酸铍（BeSO4）中。培养20小时后，通过台盼蓝染料排斥法测定细胞活力。测试的毒性最大的物质是BeSO4，其次是500摄氏度煅烧的BeO，然后是1000摄氏度煅烧的BeO。采用体外溶解技术测量BeO颗粒的相对溶解度。与1000摄氏度制备的BeO相比，500摄氏度制备的BeO具有更高的溶解度。本研究通过研究铍化合物对犬PAM的影响，并将PAM细胞毒性与铍化合物溶解度的测量值相关联，扩展了先前的工作。