Fan Yapeng, Lu Xuke, Chen Xiugui, Wang Junjuan, Wang Delong, Wang Shuai, Guo Lixue, Rui Cun, Zhang Yuexin, Cui Ruifeng, Malik Waqar Afzal, Wang Qinqin, Chen Chao, Yu John Z, Ye Wuwei
State Key Laboratory of Cotton Biology / Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Zhengzhou University / Key Laboratory for Cotton Genetic Improvement, MOA, Anyang, Henan, 455000, China.
Crop Germplasm Research Unit, Southern Plains Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), College Station, TX 77845, USA.
Genomics. 2021 May;113(3):1157-1169. doi: 10.1016/j.ygeno.2021.02.022. Epub 2021 Mar 6.
Alkaline stress is one of the abiotic stresses limiting cotton production. Though RNA-Seq analyses, have been conducted to investigate genome-wide gene expression in response to alkaline stress in plants, the response of sodium bicarbonate (NaHCO) stress-related genes in cotton has not been reported. To explore the mechanisms of cotton response to this alkaline stress, we used next-generation sequencing (NGS) technology to study transcriptional changes of cotton under NaHCO alkaline stress. A total of 18,230 and 11,177 differentially expressed genes (DEGs) were identified in cotton roots and leaves, respectively. Gene ontology (GO) analysis indicated the enrichment of DEGs involved in various stimuli or stress responses. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that DEGs associated with plant hormone signal transduction, amino acid biosynthesis, and biosynthesis of secondary metabolites were regulated in response to the NaHCO stress. We further analyzed genes enriched in secondary metabolic pathways and found that secondary metabolites were regulated to eliminate the reactive oxygen species (ROS) and improve the cotton tolerance to the NaHCO stress. In this study, we learned that the toxic effect of NaHCO was more profound than that of NaOH at the same pH. Thus, Na, HCO and pH had a great impact on the growth of cotton plant. The novel biological pathways and candidate genes for the cotton tolerance to NaHCO stress identified from the study would be useful in the genetic improvement of the alkaline tolerance in cotton.
碱胁迫是限制棉花产量的非生物胁迫之一。尽管已经通过RNA测序分析来研究植物中响应碱胁迫的全基因组基因表达，但棉花中与碳酸氢钠（NaHCO₃）胁迫相关基因的响应尚未见报道。为了探究棉花对这种碱胁迫的响应机制，我们使用下一代测序（NGS）技术研究了棉花在NaHCO₃碱胁迫下的转录变化。在棉花根和叶中分别鉴定出总共18230个和11177个差异表达基因（DEG）。基因本体论（GO）分析表明参与各种刺激或胁迫响应的DEG富集。京都基因与基因组百科全书（KEGG）通路分析表明，与植物激素信号转导、氨基酸生物合成和次生代谢物生物合成相关的DEG在响应NaHCO₃胁迫时受到调控。我们进一步分析了富集在次生代谢途径中的基因，发现次生代谢物受到调控以消除活性氧（ROS）并提高棉花对NaHCO₃胁迫的耐受性。在本研究中，我们了解到在相同pH值下，NaHCO₃的毒性作用比NaOH更显著。因此，Na⁺、HCO₃⁻和pH值对棉花植株的生长有很大影响。从该研究中鉴定出的棉花对NaHCO₃胁迫耐受性的新生物途径和候选基因将有助于棉花耐碱性的遗传改良。
