Li Juanli, Zhang Zhongxing, Xian Xulin, Zhang Donghai, Zhao Wenbing, Wang Yanxiu
Fruit Tree Physiology Lab, College of Horticulture, Gansu Agricultural University, Lanzhou, 730070, Gansu, China.
Plant Cell Rep. 2025 Aug 12;44(9):198. doi: 10.1007/s00299-025-03589-5.
MdMGT6 has a positive regulatory effect on A. thaliana, tobacco and apple calli under saline-alkali stress. As a major apple (Malus domestica) producing area, soil salinization is one of the important factors affecting apple yield in Northwest China. MGT (Magnesium transporter protein) family genes play a key role in plant growth and development and abiotic stress response. In this study, a total of 12 genes were identified by genome-wide identification of apple. Construct a phylogenetic tree among different species and divide all members into six subgroups. There are multiple stress-related regulatory elements in the MdMGTS promoter region, suggesting that it may be involved in abiotic stress. The qRT-PCR results showed that MdMGT6 was significantly highly expressed under saline-alkali stress. Despite this discovery, the mechanism of how MdMGT6 responds to saline-alkali stress has not been explored. To investigate this question, the MdMGT6 (MD10G1056700) gene were successfully cloned from Malus domestica and genetically transformed it in Arabidopsis thaliana, tobacco and apple calli. The resistance to saline-alkali stress was identified by a series of physiological and biochemical experiments. The results showed that compared with the wild type, the chlorophyll, Proline (Pro) content and antioxidant enzyme activity of the transgenic plants were significantly increased under saline-alkali stress, while the Malondialdehyde (MAD) and Relative conductivity (REC) content were decreased. In addition, saline-alkali stress related genes were significantly higher in the overexpression lines than in the WT lines. These results indicate that MdMGT6 can respond to saline-alkali stress and play a positive regulation role.
MdMGT6在盐碱胁迫下对拟南芥、烟草和苹果愈伤组织具有正向调控作用。作为主要的苹果（Malus domestica）产区，土壤盐渍化是影响中国西北苹果产量的重要因素之一。MGT（镁转运蛋白）家族基因在植物生长发育和非生物胁迫响应中起关键作用。在本研究中，通过对苹果进行全基因组鉴定共鉴定出12个基因。构建不同物种间的系统发育树并将所有成员分为六个亚组。MdMGTS启动子区域存在多个与胁迫相关的调控元件，表明它可能参与非生物胁迫。qRT-PCR结果表明，MdMGT6在盐碱胁迫下显著高表达。尽管有这一发现，但MdMGT6响应盐碱胁迫的机制尚未得到探索。为研究这个问题，成功从苹果（Malus domestica）中克隆出MdMGT6（MD10G1056700）基因，并在拟南芥、烟草和苹果愈伤组织中进行遗传转化。通过一系列生理生化实验鉴定其对盐碱胁迫的抗性。结果表明，与野生型相比，转基因植株在盐碱胁迫下叶绿素、脯氨酸（Pro）含量和抗氧化酶活性显著增加，而丙二醛（MAD）和相对电导率（REC）含量降低。此外，过表达株系中盐碱胁迫相关基因显著高于野生型株系。这些结果表明，MdMGT6能够响应盐碱胁迫并发挥正向调控作用。
