Laboratory of Biotechnology, Institute of Natural Sciences and Mathematics, Ural Federal University, Ekaterinburg, 620002, Russia.
Department of Experimental Biology and Biotechnology, Institute of Natural Sciences and Mathematics, Ural Federal University, Ekaterinburg, 620002, Russia.
Chemosphere. 2021 Aug;276:130038. doi: 10.1016/j.chemosphere.2021.130038. Epub 2021 Feb 22.
Copper (Cu) is an essential element, however it's excess into the environment causes detrimental effect on plant and risks for public health. Four Cu and drought tolerant 1-aminocyclopropane-1-carboxylate (ACC) deaminase producing rhizobacteria were isolated from the roots of Trifolium repens L. growing on Cu smelter contaminated soils, characterized and identified based on 16S rRNA gene sequencing. A consortium of high ACC deaminase (53.74 μM α-ketobutyrate mg protein h) producing bacteria Pseudomonas sp. strain TR15a + siderophore producing Bacillus aerophilus strain TR15c significantly (p < 0.05) produced better results for multiple-metal tolerance including Cu (1750 mg kg), antibiotic resistance (ampicillin, kanamycin, chloramphenicol, penicillin, tetracycline, and streptomycin) and plant growth promoting attributes (phosphate solubilization: 315 mg L, indole-3-acetic acid (IAA) production: 8 mg L, ammonia and hydrogen cyanide production) as compared to individual isolates. Pot scale experiment (enriched with 100 mg Cu kg) showed inoculation of Helianthus annuus seeds with consortium of TR15a + TR15c had significantly (p < 0.05) improved seed germination by 32%, total dry biomass by 64%, root Cu by 47% and shoot Cu by 75% as compared to uninoculated control whereas 0.2-7 fold higher results were observed for above stated parameters as compared to four individual isolates studied. The result suggests consortium of ACC deaminase producing Pseudomonas sp. TR15a and siderophore producing B. aerophilus TR15c could play a vital role in enhanced Cu uptake and improvement of biomass and may provide a better alternative for decontamination of Cu contaminated natural ecosystem than individual isolates.
铜（Cu）是一种必需元素，但其在环境中的过量存在会对植物造成有害影响，并对公众健康构成风险。从生长在铜冶炼厂污染土壤上的三叶草（Trifolium repens L.）根部分离出 4 株耐铜和耐旱的 1-氨基环丙烷-1-羧酸（ACC）脱氨酶产生的根际细菌，根据 16S rRNA 基因测序进行了特征和鉴定。高 ACC 脱氨酶（53.74μM α-酮丁酸 mg 蛋白 h）产生菌假单胞菌（Pseudomonas）TR15a+铁载体产生枯草芽孢杆菌（Bacillus aerophilus）TR15c 的 consortium 显著（p<0.05）产生了更好的结果，包括对多种金属的耐受性，包括 Cu（1750mg kg）、抗生素抗性（氨苄青霉素、卡那霉素、氯霉素、青霉素、四环素和链霉素）和植物生长促进特性（溶磷：315mg L、吲哚-3-乙酸（IAA）产生：8mg L、氨和氢氰酸产生）与单个分离株相比。盆栽实验（添加 100mg Cu kg）表明，用 TR15a+TR15c consortium接种向日葵种子，与未接种对照相比，种子发芽率显著提高 32%，总干生物量提高 64%，根 Cu 提高 47%，茎 Cu 提高 75%，而与上述四个单独研究的分离株相比，上述参数的结果提高了 0.2-7 倍。结果表明，ACC 脱氨酶产生菌假单胞菌 TR15a 和铁载体产生菌枯草芽孢杆菌 TR15c 的 consortium 可以在增强 Cu 吸收和提高生物量方面发挥重要作用，并可能为污染 Cu 的自然生态系统的净化提供比单个分离株更好的选择。
