Department of Biosciences, UGC-Centre of Advanced Study, Sardar Patel University, Satellite Campus, Vadtal Road, Bakrol, 388 315, Anand, Gujarat, India.
Department of Biosciences, UGC-Centre of Advanced Study, Sardar Patel University, Satellite Campus, Vadtal Road, Bakrol, 388 315, Anand, Gujarat, India.
Microbiol Res. 2021 Jul;248:126734. doi: 10.1016/j.micres.2021.126734. Epub 2021 Mar 1.
The diseases caused by phytopathogens account for huge economic losses in the agricultural sector. Paenibacillus polymyxa is one of the agriculturally important biocontrol agents and plant growth promoting bacterium. This study describes the antifungal potential of P. polymyxa HK4 against an array of fungal phytopathogens and its ability to stimulate seed germination of cumin and groundnut under in vitro conditions. The cumin and groundnut seeds bacterized with HK4 exhibited enhanced germination efficiency in comparison to controls. The use of HK4 as a soil inoculant significantly promoted the shoot length and fresh weight of groundnut plants in pot studies. The draft genome analysis of HK4 revealed the genetic attributes for motility, root colonization, antagonism, phosphate solubilization, siderophore production and production of volatile organic compounds. The bacterium HK4 harnessed several hydrolytic enzymes that may assist its competence in the rhizosphere. The PCR amplification and sequence analysis of the conserved region of the fusA gene amplicon revealed the ability of HK4 to produce fusaricidin. Furthermore, the LC-ESI-MS/MS of crude cell pellet extract of HK4 confirmed the presence of fusaricidin as a major antifungal metabolite. This study demonstrated the potential of HK4 as a biocontrol agent and a plant growth promoter.
植物病原菌引起的疾病给农业部门造成了巨大的经济损失。多粘类芽孢杆菌是一种重要的农业生物防治剂和植物生长促进细菌。本研究描述了多粘类芽孢杆菌 HK4 对一系列真菌病原菌的抗真菌潜力及其在体外条件下刺激孜然和花生种子发芽的能力。与对照相比，用 HK4 处理的孜然和花生种子的发芽效率显著提高。在盆栽研究中，将 HK4 用作土壤接种剂可显著促进花生植株的茎长和鲜重。HK4 的基因组草图分析揭示了其运动性、根定植、拮抗作用、解磷作用、铁载体生产和挥发性有机化合物生产的遗传特性。该细菌 HK4 利用了几种水解酶，这可能有助于其在根际中的竞争力。fusA 基因扩增子保守区的 PCR 扩增和序列分析显示 HK4 具有产生 Fusaricidin 的能力。此外，HK4 粗细胞沉淀提取物的 LC-ESI-MS/MS 分析证实了 Fusaricidin 作为主要抗真菌代谢物的存在。本研究表明了 HK4 作为生物防治剂和植物生长促进剂的潜力。
