State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang 550025, China.
Pestic Biochem Physiol. 2018 May;147:67-74. doi: 10.1016/j.pestbp.2017.08.012. Epub 2017 Aug 15.
Dendrobium nobile (D. nobile) is a valuable Chinese herbal medicine. The discovery of microbial resources from has provided a wealth of raw materials. Stalk rot, which is caused by Pestalotiopsis, is one of the most serious diseases of D nobile and has resulted in serious losses in production. However, an effective method for the prevention and control of stalk rot remains lacking. In this study, we aimed to identify a biocontrol strain against Pestalotiopsis. We isolated Paenibacillus polymyxa Y-1, an endophytic bacterium, from the stem of D. nobile. Three pairs of active metabolites isolated from this bacterium were identified as fusaricidin compounds. We then investigated the mechanism of fusaricidin compounds on Pestalotiopsis via proteomics. Proteomics data showed that the compounds mainly inhibit energy generation in the respiratory chain and amino acid biosynthesis of Pestalotiopsis.
铁皮石斛（D. nobile）是一种有价值的中药。从其中发现的微生物资源为提供了丰富的原材料。茎腐病是铁皮石斛最严重的病害之一，导致严重的产量损失。然而，目前仍缺乏有效的防治方法。本研究旨在筛选对茎腐病具有生防作用的菌株。我们从铁皮石斛的茎中分离到一种内生细菌，命名为多粘类芽孢杆菌 Y-1。从该细菌中分离到的三种活性代谢产物被鉴定为 Fusaricidin 类化合物。然后，我们通过蛋白质组学研究 Fusaricidin 化合物对 Pestalotiopsis 的作用机制。蛋白质组学数据表明，这些化合物主要抑制 Pestalotiopsis 呼吸链中的能量生成和氨基酸生物合成。
