Institute of Resources, Environment and Soil Fertilizer, Fujian Academy of Agricultural Sciences, Wusi Road 247, Fuzhou, 350003, People's Republic of China.
Institute of Food Science and Technology, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, People's Republic of China.
World J Microbiol Biotechnol. 2024 Oct 3;40(11):332. doi: 10.1007/s11274-024-04139-z.
Brevibacillus brevis FJAT-0809-GLX has a broad spectrum of antimicrobial activity. Understanding the molecular basis of biocontrol ability of B. brevis will allow us to develop effective microbial agents for sustainable agriculture. In this study, we present the complete and annotated genome sequence of FJAT-0809-GLX. The complete genome size of B. brevis FJAT-0809-GLX was 6,137,019 bp, with 5688 predicted coding sequences (CDS). The average GC content of 47.38%, and there were 44 copies of the rRNAs operon (16S, 23S and 5S RNA), and 127 tRNA genes. A total of 11,162 genes were functionally annotated with the COG, GO, and KEGG databases, and 123 genes belonged to CAZymes. Genomic secondary metabolite analysis indicated 13 clusters encoding potential new antimicrobials. FJAT-0809-GLX was designated as B. brevis according to average nucleotide polymorphism (ANI) and phylogenetic analysis. The pangenome consisted of 7141 homologous genes, and 4469 homologous genes shared by B. brevis FJAT-0809-GLX, B. brevis NBRC100599, B. brevis DSM30, and B. brevis NCTC2611. The number of unique homologous genes of B. brevis FJAT-0809-GLX (419 genes) and B. brevis NBRC100599 (480 genes) were much more than those in B. brevis DSM30 (13 genes), and B. brevis NCTC2611 (6 genes). Nine gene clusters encoding for secondary metabolite biosynthesis were compared in the genome of B. brevis FJAT-0809-GLX with those of B. brevis NBRC100599, B. brevis DSM30 and B. brevis NCTC2611, and the gene clusters encoding for lantipeptide and transatpks-otherks only existed in genome of B. brevis FJAT-0809-GLX. The 11 BbPks genes were included in the B. brevis FJAT-0809-GLX genome, which contained the conserved PS-DH domain. The relative expression of BbPksL, BbPksM2, BbPksM3, BbPksN3, BbPksN4 and BbPksN5 reached a maximum at 120 h and then decreased at 144 h. Our results provided detailed genomic and Pks genes information for the FJAT-0809-GLX strain, and lid a foundation for studying its biocontrol mechanisms.
短芽孢杆菌 FJAT-0809-GLX 具有广谱的抗菌活性。了解短芽孢杆菌生防能力的分子基础，将使我们能够开发出用于可持续农业的有效微生物制剂。在本研究中，我们呈现了 FJAT-0809-GLX 的完整注释基因组序列。短芽孢杆菌 FJAT-0809-GLX 的完整基因组大小为 6137019bp，预测有 5688 个编码序列（CDS）。平均 GC 含量为 47.38%，有 44 个 rRNA 操纵子（16S、23S 和 5S RNA）和 127 个 tRNA 基因。总共对 11162 个基因进行了 COG、GO 和 KEGG 数据库的功能注释，其中 123 个基因属于 CAZymes。基因组次生代谢产物分析表明，有 13 个簇编码潜在的新抗菌药物。FJAT-0809-GLX 根据平均核苷酸一致性（ANI）和系统发育分析被指定为短芽孢杆菌。泛基因组由 7141 个同源基因组成，短芽孢杆菌 FJAT-0809-GLX、短芽孢杆菌 NBRC100599、短芽孢杆菌 DSM30 和短芽孢杆菌 NCTC2611 共享 4469 个同源基因。短芽孢杆菌 FJAT-0809-GLX（419 个）和短芽孢杆菌 NBRC100599（480 个）的特有同源基因数量远多于短芽孢杆菌 DSM30（13 个）和短芽孢杆菌 NCTC2611（6 个）。在短芽孢杆菌 FJAT-0809-GLX 的基因组中，比较了 9 个编码次生代谢产物生物合成的基因簇与短芽孢杆菌 NBRC100599、短芽孢杆菌 DSM30 和短芽孢杆菌 NCTC2611 的基因组，只有短芽孢杆菌 FJAT-0809-GLX 的基因组中存在编码 lantipeptide 和 transatpks-otherks 的基因簇。在短芽孢杆菌 FJAT-0809-GLX 基因组中包含 11 个 BbPks 基因，其中包含保守的 PS-DH 结构域。BbPksL、BbPksM2、BbPksM3、BbPksN3、BbPksN4 和 BbPksN5 的相对表达在 120h 时达到最大值，然后在 144h 时下降。我们的结果为 FJAT-0809-GLX 菌株提供了详细的基因组和 Pks 基因信息，为研究其生防机制奠定了基础。
