• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Bri2 BRICHOS 涂层磁性颗粒的合成与表征及其在蛋白质钓取中的应用：新型结合蛋白的鉴定。

The synthesis and characterization of Bri2 BRICHOS coated magnetic particles and their application to protein fishing: Identification of novel binding proteins.

机构信息

School of Natural Sciences and Health, Tallinn University, Tallinn, Estonia.

Department of Biosciences and Nutrition, Karolinska Institutet, 14183, Huddinge, Sweden.

出版信息

J Pharm Biomed Anal. 2021 May 10;198:113996. doi: 10.1016/j.jpba.2021.113996. Epub 2021 Feb 26.

DOI:10.1016/j.jpba.2021.113996
PMID:33690096
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10644258/
Abstract

Human integral membrane protein 2B (ITM2B or Bri2) is a member of the BRICHOS family, proteins that efficiently prevent Aβ42 aggregation via a unique mechanism. The identification of novel Bri2 BRICHOS client proteins could help elucidate signaling pathways and determine novel targets to prevent or cure amyloid diseases. To identify Bri2 BRICHOS interacting partners, we carried out a 'protein fishing' experiment using recombinant human (rh) Bri2 BRICHOS-coated magnetic particles, which exhibit essentially identical ability to inhibit Aβ42 fibril formation as free rh Bri2 BRICHOS, in combination with proteomic analysis on homogenates of SH-SY5Y cells. We identified 70 proteins that had more significant interactions with rh Bri2 BRICHOS relative to the corresponding control particles. Three previously identified Bri2 BRICHOS interacting proteins were also identified in our 'fishing' experiments. The binding affinity of Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), the top 'hit', was calculated and was identified as a strong interacting partner. Enrichment analysis of the retained proteins identified three biological pathways: Rho GTPase, heat stress response and pyruvate, cysteine and methionine metabolism.

摘要

人 integral 膜蛋白 2B (ITM2B 或 Bri2) 是 BRICHOS 家族的成员，该家族的蛋白通过独特的机制有效地阻止 Aβ42 聚集。鉴定新的 Bri2 BRICHOS 客户蛋白可以帮助阐明信号通路，并确定预防或治疗淀粉样变性疾病的新靶点。为了鉴定 Bri2 BRICHOS 相互作用蛋白，我们使用重组人 (rh) Bri2 BRICHOS 涂层的磁性颗粒进行了“蛋白钓饵”实验，该颗粒与游离 rh Bri2 BRICHOS 相比，具有基本相同的抑制 Aβ42 纤维形成的能力，结合 SH-SY5Y 细胞匀浆的蛋白质组学分析。我们鉴定出 70 种与 rh Bri2 BRICHOS 相互作用比相应对照颗粒更显著的蛋白。我们的“钓饵”实验还鉴定出了三种先前鉴定出的 Bri2 BRICHOS 相互作用蛋白。计算了 top“hit”甘油醛 3-磷酸脱氢酶 (GAPDH) 的结合亲和力，并将其鉴定为强相互作用伙伴。保留蛋白的富集分析确定了三个生物学途径：Rho GTPase、热应激反应和丙酮酸、半胱氨酸和蛋氨酸代谢。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12e1/10644258/570c0f50575e/nihms-1804145-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12e1/10644258/d085b8ddbe02/nihms-1804145-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12e1/10644258/82426702f19a/nihms-1804145-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12e1/10644258/ab236cb2920a/nihms-1804145-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12e1/10644258/570c0f50575e/nihms-1804145-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12e1/10644258/d085b8ddbe02/nihms-1804145-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12e1/10644258/82426702f19a/nihms-1804145-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12e1/10644258/ab236cb2920a/nihms-1804145-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12e1/10644258/570c0f50575e/nihms-1804145-f0004.jpg

相似文献

1
The synthesis and characterization of Bri2 BRICHOS coated magnetic particles and their application to protein fishing: Identification of novel binding proteins.Bri2 BRICHOS 涂层磁性颗粒的合成与表征及其在蛋白质钓取中的应用：新型结合蛋白的鉴定。
J Pharm Biomed Anal. 2021 May 10;198:113996. doi: 10.1016/j.jpba.2021.113996. Epub 2021 Feb 26.
2
Identification of glycolytic proteins as binding partners of Bri2 BRICHOS domain.鉴定糖酵解蛋白作为 Bri2 BRICHOS 结构域的结合伴侣。
J Pharm Biomed Anal. 2023 Aug 5;232:115465. doi: 10.1016/j.jpba.2023.115465. Epub 2023 May 18.
3
Identification of cytoskeletal proteins as binding partners of Bri2 BRICHOS domain.鉴定细胞骨架蛋白作为 Bri2 BRICHOS 结构域的结合伴侣。
Mol Cell Neurosci. 2023 Jun;125:103843. doi: 10.1016/j.mcn.2023.103843. Epub 2023 Mar 17.
4
Blood-brain and blood-cerebrospinal fluid passage of BRICHOS domains from two molecular chaperones in mice.两种分子伴侣在小鼠体内的 BRICHOS 结构域通过血脑屏障和血脑脊液屏障。
J Biol Chem. 2019 Feb 22;294(8):2606-2615. doi: 10.1074/jbc.RA118.004538. Epub 2018 Dec 31.
5
Recombinant BRICHOS chaperone domains delivered to mouse brain parenchyma by focused ultrasound and microbubbles are internalized by hippocampal and cortical neurons.经聚焦超声和微泡递送至鼠脑实质的重组 BRICHOS 伴侣结构域被海马和皮质神经元内化。
Mol Cell Neurosci. 2020 Jun;105:103498. doi: 10.1016/j.mcn.2020.103498. Epub 2020 May 8.
6
Determinants for Substoichiometric Inhibition of IAPP and Aβ Amyloid Aggregations by Bri2 BRICHOS.Bri2 BRICHOS对胰岛淀粉样多肽（IAPP）和β淀粉样蛋白（Aβ）淀粉样聚集的亚化学计量抑制的决定因素
ACS Chem Neurosci. 2025 Mar 19;16(6):1150-1160. doi: 10.1021/acschemneuro.4c00839. Epub 2025 Mar 4.
7
The Bri2 and Bri3 BRICHOS Domains Interact Differently with Aβ and Alzheimer Amyloid Plaques.Bri2和Bri3的BRICHOS结构域与Aβ及阿尔茨海默病淀粉样斑块的相互作用方式不同。
J Alzheimers Dis Rep. 2018 Feb 16;2(1):27-39. doi: 10.3233/ADR-170051.
8
BRICHOS domain of Bri2 inhibits islet amyloid polypeptide (IAPP) fibril formation and toxicity in human beta cells.Bri2 的 BRICHOS 结构域抑制人胰岛β细胞中胰岛淀粉样多肽（IAPP）纤维的形成和毒性。
Proc Natl Acad Sci U S A. 2018 Mar 20;115(12):E2752-E2761. doi: 10.1073/pnas.1715951115. Epub 2018 Mar 5.
9
Recombinant Bri3 BRICHOS domain is a molecular chaperone with effect against amyloid formation and non-fibrillar protein aggregation.重组 Bri3 BRICHOS 结构域是一种具有抗淀粉样形成和非纤维状蛋白聚集作用的分子伴侣。
Sci Rep. 2020 Jun 17;10(1):9817. doi: 10.1038/s41598-020-66718-y.
10
Dementia-related Bri2 BRICHOS is a versatile molecular chaperone that efficiently inhibits Aβ42 toxicity in Drosophila.与痴呆症相关的Bri2 BRICHOS是一种多功能分子伴侣，可有效抑制果蝇中的Aβ42毒性。
Biochem J. 2016 Oct 15;473(20):3683-3704. doi: 10.1042/BCJ20160277. Epub 2016 Aug 11.

引用本文的文献

1
Identification of glycolytic proteins as binding partners of Bri2 BRICHOS domain.鉴定糖酵解蛋白作为 Bri2 BRICHOS 结构域的结合伴侣。
J Pharm Biomed Anal. 2023 Aug 5;232:115465. doi: 10.1016/j.jpba.2023.115465. Epub 2023 May 18.

本文引用的文献

1
Smallest Secondary Nucleation Competent Aβ Aggregates Probed by an ATP-Independent Molecular Chaperone Domain.小分子非成核性 Aβ 聚集物的探测：一种不依赖于 ATP 的分子伴侣结构域。
Biochemistry. 2021 Mar 9;60(9):678-688. doi: 10.1021/acs.biochem.1c00003. Epub 2021 Feb 23.
2
Recombinant Bri3 BRICHOS domain is a molecular chaperone with effect against amyloid formation and non-fibrillar protein aggregation.重组 Bri3 BRICHOS 结构域是一种具有抗淀粉样形成和非纤维状蛋白聚集作用的分子伴侣。
Sci Rep. 2020 Jun 17;10(1):9817. doi: 10.1038/s41598-020-66718-y.
3
Heparan sulfate is a clearance receptor for aberrant extracellular proteins.
硫酸乙酰肝素是一种清除异常细胞外蛋白的受体。
J Cell Biol. 2020 Mar 2;219(3). doi: 10.1083/jcb.201911126.
4
Augmentation of Bri2 molecular chaperone activity against amyloid-β reduces neurotoxicity in mouse hippocampus in vitro.Bri2 分子伴侣活性增强可减少体外小鼠海马体中淀粉样β的神经毒性。
Commun Biol. 2020 Jan 20;3(1):32. doi: 10.1038/s42003-020-0757-z.
5
Physical Activity Associated Proteomics of Skeletal Muscle: Being Physically Active in Daily Life May Protect Skeletal Muscle From Aging.骨骼肌的体力活动相关蛋白质组学：在日常生活中保持身体活跃可能保护骨骼肌免于衰老。
Front Physiol. 2019 Mar 26;10:312. doi: 10.3389/fphys.2019.00312. eCollection 2019.
6
The proteostasis network and its decline in ageing.蛋白质稳态网络及其在衰老过程中的衰退。
Nat Rev Mol Cell Biol. 2019 Jul;20(7):421-435. doi: 10.1038/s41580-019-0101-y.
7
Amyloid nomenclature 2018: recommendations by the International Society of Amyloidosis (ISA) nomenclature committee.淀粉样变命名 2018：国际淀粉样变学会（ISA）命名委员会的建议。
Amyloid. 2018 Dec;25(4):215-219. doi: 10.1080/13506129.2018.1549825. Epub 2019 Jan 7.
8
Heat Shock Proteins Regulatory Role in Neurodevelopment.热休克蛋白在神经发育中的调节作用。
Front Neurosci. 2018 Nov 12;12:821. doi: 10.3389/fnins.2018.00821. eCollection 2018.
9
The Bri2 and Bri3 BRICHOS Domains Interact Differently with Aβ and Alzheimer Amyloid Plaques.Bri2和Bri3的BRICHOS结构域与Aβ及阿尔茨海默病淀粉样斑块的相互作用方式不同。
J Alzheimers Dis Rep. 2018 Feb 16;2(1):27-39. doi: 10.3233/ADR-170051.
10
BRICHOS domain of Bri2 inhibits islet amyloid polypeptide (IAPP) fibril formation and toxicity in human beta cells.Bri2 的 BRICHOS 结构域抑制人胰岛β细胞中胰岛淀粉样多肽（IAPP）纤维的形成和毒性。
Proc Natl Acad Sci U S A. 2018 Mar 20;115(12):E2752-E2761. doi: 10.1073/pnas.1715951115. Epub 2018 Mar 5.