Appl Opt. 2021 Mar 1;60(7):2069-2076. doi: 10.1364/AO.415270.
High-resolution imaging using high numerical aperture imaging optics is commonly known to cause a narrow depth of focus, which limits the depth of field in optical coherence tomography (OCT). To achieve semi-invariant high resolution in all directions, Gabor domain optical coherence microscopy (GD-OCM) combines the in-focus regions of multiple cross-sectional images that are acquired while shifting the focal plane of the objective lens. As a result, GD-OCM requires additional processes for in-focus extraction and fusion, leading to longer processing times, as compared with conventional frequency domain OCT (FD-OCT). We previously proposed a method of spectral domain Gabor fusion that has been proven to improve the processing speed of GD-OCM. To investigate the full potential of the spectral domain Gabor fusion technique, we present the implementation of the spectral domain Gabor fusion algorithm using field programmable gate arrays (FPGAs) in a spectral acquisition hardware device. All filtering processes are now performed in an acquisition device as opposed to the post-processing of the original GD-OCM, which reduces the amount of data transfer between the image acquisition device and the processing host. To clearly demonstrate the imaging performance of the implemented system, we performed GD-OCM imaging of a stack of polymeric tapes. GD-OCM imaging was performed over seven focus zones. The results showed that the processing time for linear wavenumber calibration and spectral Gabor filtering can be improved with FPGA implementation. The total processing time was improved by about 35%.
使用高数值孔径成像光学元件进行高分辨率成像是众所周知的,会导致焦点深度变窄,从而限制光学相干断层扫描(OCT)的景深。为了在所有方向上实现半不变高分辨率,Gabor 域光学相干显微镜(GD-OCM)结合了在获取物镜焦平面移动时获取的多个横截面图像的聚焦区域。因此,与传统的频域 OCT(FD-OCT)相比,GD-OCM 需要额外的聚焦提取和融合过程,导致处理时间更长。我们之前提出了一种光谱域 Gabor 融合方法,该方法已被证明可以提高 GD-OCM 的处理速度。为了充分发挥光谱域 Gabor 融合技术的潜力,我们在光谱采集硬件设备中使用现场可编程门阵列(FPGA)实现了光谱域 Gabor 融合算法。现在,所有滤波过程都在采集设备中执行,而不是在原始 GD-OCM 的后处理中执行,这减少了图像采集设备和处理主机之间的数据传输量。为了清楚地展示所实现系统的成像性能,我们对堆叠的聚合物带进行了 GD-OCM 成像。GD-OCM 成像在七个焦点区域进行。结果表明,FPGA 实现可以提高线性波数校准和光谱 Gabor 滤波的处理时间。总处理时间提高了约 35%。