Liu Siyang, Mulligan Jeffrey A, Adie Steven G
School of Electrical and Computer Engineering, Cornell University, Ithaca, NY 14853, USA.
Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY 14853, USA.
Biomed Opt Express. 2018 Jun 15;9(7):3137-3152. doi: 10.1364/BOE.9.003137. eCollection 2018 Jul 1.
Optical coherence microscopy (OCM) is a promising modality for high resolution imaging, but has limited ability to capture large-scale volumetric information about dynamic biological processes with cellular resolution. To enhance the throughput of OCM, we implemented a hybrid adaptive optics (hyAO) approach that combines computational adaptive optics with an intentionally aberrated imaging beam generated via hardware adaptive optics. Using hyAO, we demonstrate the depth-equalized illumination and collection ability of an astigmatic beam compared to a Gaussian beam for cellular-resolution imaging. With this advantage, we achieved volumetric OCM with a higher space-bandwidth- product compared to Gaussian-beam acquisition that employed focus-scanning across depth. HyAO was also used to perform volumetric time-lapse OCM imaging of cellular dynamics over a 1mm × 1mm × 1mm field-of-view with 2 μm isotropic spatial resolution and 3-minute temporal resolution. As hyAO is compatible with both spectral-domain and swept-source beam-scanning OCM systems, significant further improvements in absolute volumetric throughput are possible by use of ultrahigh-speed swept sources.
光学相干显微镜（OCM）是一种很有前景的高分辨率成像方式，但在以细胞分辨率捕获有关动态生物过程的大规模体积信息方面能力有限。为了提高OCM的通量，我们实施了一种混合自适应光学（hyAO）方法，该方法将计算自适应光学与通过硬件自适应光学产生的有意像差成像光束相结合。使用hyAO，我们展示了与高斯光束相比，像散光束在细胞分辨率成像中的深度均衡照明和采集能力。凭借这一优势，与采用跨深度聚焦扫描的高斯光束采集相比，我们实现了具有更高空间带宽积的体积OCM。HyAO还用于在1mm×1mm×1mm的视场中对细胞动力学进行体积延时OCM成像，具有2μm各向同性空间分辨率和3分钟时间分辨率。由于hyAO与光谱域和扫频源光束扫描OCM系统都兼容，通过使用超高速扫频源，绝对体积通量有可能得到显著进一步提高。
