Jiangsu Key Laboratory of Anaerobic Biotechnology, School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China; Jiangsu Collaborative Innovation Center of Water Treatment Technology and Material, Suzhou 215011, China.
Jiangsu Key Laboratory of Anaerobic Biotechnology, School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China.
Bioresour Technol. 2021 Jun;329:124924. doi: 10.1016/j.biortech.2021.124924. Epub 2021 Mar 3.
For exploring the impact of temperature on antibiotic resistant genes (ARGs) during sludge anaerobic digestion (AD), the dynamic variations of sludge ARGs, plasmid ARGs, and cell-free ARGs in mesophilic (25 °C and 35 °C) and thermophilic (55 °C) digesters were investigated. The study revealed that the abundance of sludge ARGs and plasmid ARGs in thermophilic sludge AD was significantly lower than that in mesophilic digesters, while the cell-free ARGs abundance of the thermophilic digesters was similar to mesophilic digesters. Higher archaea abundance, lower bacteria abundance, and different microbial community were found in thermophilic digesters compared to that of mesophilic ones. Firmicutes might be a main group of potential hosts of ARGs in sludge AD. The distinct microbial community was the main contributor to the low ARGs abundance in thermophilic sludge AD. Thermophilic operation at 55 °C rather than mesophilic operation is more conducive to control ARGs in sludge anaerobic digestion.
为了探究温度变化对污泥厌氧消化（AD）过程中抗生素抗性基因（ARGs）的影响，本研究分别在中温（25°C 和 35°C）和高温（55°C）条件下，对 ARGs、质粒 ARGs 和游离态 ARGs 在污泥中的动态变化进行了研究。研究结果表明，高温条件下污泥 AD 中污泥 ARGs 和质粒 ARGs 的丰度明显低于中温消化器，而游离态 ARGs 的丰度与中温消化器相似。与中温消化器相比，高温消化器中的古菌丰度更高，细菌丰度更低，微生物群落也存在差异。在污泥 AD 中，厚壁菌门可能是 ARGs 的主要潜在宿主。不同的微生物群落是导致高温污泥 AD 中 ARGs 丰度较低的主要原因。相比中温运行，55°C 的高温运行更有利于控制污泥厌氧消化过程中的 ARGs。
