好氧和嗜热厌氧消化化学强化一级处理（CEPT）污泥中抗生素耐药基因的命运
Fate of antibiotic resistance genes in mesophilic and thermophilic anaerobic digestion of chemically enhanced primary treatment (CEPT) sludge.
机构信息
School of Earth Sciences and Environmental Engineering, Gwangju Institute of Science and Technology, Buk-gu, Gwangju 500-712, Republic of Korea.
School of Environmental Science and Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk, Republic of Korea.
出版信息
Bioresour Technol. 2017 Nov;244(Pt 1):433-444. doi: 10.1016/j.biortech.2017.07.153. Epub 2017 Jul 29.
Anaerobic digestion (AD) of chemically enhanced primary treatment (CEPT) sludge and non-CEPT (conventional sedimentation) sludge were comparatively operated under mesophilic and thermophilic conditions. The highest methane yield (692.46±0.46mL CH/g VS in CEPT sludge) was observed in mesophilic AD of CEPT sludge. Meanwhile, thermophilic conditions were more favorable for the removal of total antibiotic resistance genes (ARGs). In this study, no measurable difference in the fates and removal of ARGs and class 1 integrin-integrase gene (intI1) was observed between treated non-CEPT and CEPT sludge. However, redundancy analysis indicated that shifts in bacterial community were primarily accountable for the variations in ARGs and intI1. Network analysis further revealed potential host bacteria for ARGs and intI1.
采用中温和高温条件下对经过化学强化一级处理（CEPT）的污泥和未经CEPT（传统沉淀）的污泥进行厌氧消化（AD）的效果进行了比较。在 CEPT 污泥的中温 AD 中观察到了最高的甲烷产量（692.46±0.46mL CH/g VS）。同时，高温条件更有利于去除总抗生素抗性基因（ARGs）。在本研究中，在处理过的非CEPT 和 CEPT 污泥中，未观察到 ARGs 和 1 类整合子-整合酶基因（intI1）的命运和去除有可衡量的差异。然而，冗余分析表明，细菌群落的变化是 ARGs 和 intI1 变化的主要原因。网络分析进一步揭示了 ARGs 和 intI1 的潜在宿主细菌。
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