[The use of a radioimmunologic method of analysis of 11-deoxycortisol for the evaluation of the function of the pituitary-adrenal cortex system during the metopirone test].

A radioimmunoassay of II-deoxycortisol in human and animal blood plasma was developed. The antiserum used in the method was prepared against a conjugate of II-deoxycortisol-3(0-carboxymethyloxime) and bovine serum albumin. Specificity of the antiserum was determined in cross reactions using 32 steroids. High specificity of the antiserum allowed to determine II-deoxycortisol without preliminary chromatographic isolation. Sensitivity of the method enabled to detect 25 pg of II-deoxycortisol. The intra- and interassay variations were less than 10% and 15%, respectively. The method was used for evaluation of hypophysis-adrenal cortex system functions under the conditions of metopirone test.