Kobayashi Y, Mukai H, Tsubota N, Watanabe F
J Steroid Biochem. 1984 Apr;20(4A):913-5.
Enzyme immunoassay of serum 11-deoxycortisol was established by using alkaline phosphatase as a label. The labelled alkaline phosphatase was stable for at least a year. 11-Deoxycortisol in serum was purified by applying to Sephadex LH-20 column prior to present enzyme immunoassay. Separation of bound and free form was carried out by adding anti-rabbit gamma-globulin goat antiserum as a second antibody. The minimal amount of 11-deoxycortisol detected was 40 pg/tube and the measurable range was from 0.04 to 10 micrograms/dl. Intra- and interassay coefficient of variations were 3.8-4.5% and 3.4-5.3%, respectively. 11-Deoxycortisol values determined by the present method correlated well with those determined by radioimmunoassay (r = 0.98, y = 0.93 x + 0.19, n = 28). This enzyme immunoassay satisfied the standard criteria of dilution and accuracy, and is applicable to routine determination of serum 11-deoxycortisol in any clinical laboratory as a non-isotopic immunoassay.
采用碱性磷酸酶作为标记物建立了血清11-脱氧皮质醇的酶免疫测定法。标记的碱性磷酸酶至少可稳定保存一年。在进行本酶免疫测定之前，血清中的11-脱氧皮质醇通过应用于Sephadex LH-20柱进行纯化。通过加入抗兔γ-球蛋白山羊抗血清作为第二抗体来进行结合型和游离型的分离。检测到的11-脱氧皮质醇的最小量为40 pg/管，可测量范围为0.04至10微克/分升。批内和批间变异系数分别为3.8 - 4.5%和3.4 - 5.3%。用本法测定的11-脱氧皮质醇值与放射免疫测定法测定的值相关性良好（r = 0.98，y = 0.93x + 0.19，n = 28）。这种酶免疫测定法符合稀释和准确性的标准要求，作为一种非同位素免疫测定法可应用于任何临床实验室进行血清11-脱氧皮质醇的常规测定。
