[Structural changes in the lipid component of membranes of endoplasmatic reticulum in early and late periods after liver ischemia].

Total ischemia of rat liver tissue within 30 min caused distinct alterations in lipid component of endoplasmic reticulum membranes, which occurred within three weeks of the postischemic period. During this period the rate of microsomal lipids saturation was elevated due to an increase in the relative content of saturated fatty acids. Two-step alterations were observed in the patterns of fluorescent probe binding (I-amino naphthalene-8-sulfonate) as well as in sensitivity of cytochrome P-450 to the impairment in vitro after induction of lipid peroxidation in microsomal membranes. Within 1-3 days after the ischemia affinity of the fluorescent probe to microsomes was decreased, while stability of cytochrome P-450 to impairment during lipid peroxidation induction was increased. Within 7-14 days affinity of the fluorescent probe to membranes was markedly elevated and stability of cytochrome P-450 to the impairment in vitro was lowered in the reactions of lipid peroxidation.