Research Center for Analytical Sciences, Tianjin Key Laboratory of Biosensing and Molecular Recognition, College of Chemistry, Nankai University, Tianjin 300071, China.
Anal Chem. 2021 Mar 23;93(11):4975-4983. doi: 10.1021/acs.analchem.1c00281. Epub 2021 Mar 10.
Inflammatory bowel disease (IBD) is a common gastrointestinal inflammatory disease, affecting a huge number of people worldwide with increasing morbidity each year. Although the etiology of IBD has not been fully elucidated, it is understood to be closely related to upregulation of the production of NO. Herein, we first report a donor-acceptor-donor (D-A-D)-type near-infrared (NIR) fluorescent probe for real-time detection of NO in IBD by harnessing the enhanced intramolecular charge transfer mechanism. exhibited good water solubility, high photostability, and excellent NIR absorbance and emission at 700 and 750/800 nm, respectively. Moreover, it was able to sensitively and specifically detect exogenous and endogenous NO in the lysosomes of living cells. Notably, enabled to noninvasively visualize NO generation in a lipopolysaccharide-induced IBD mouse model for 30 h, showing a two- to threefold higher NIR fluorescence intensity in the intestines and feces of IBD mice than normal mice. This work demonstrates that is promising as a diagnosis agent for real-time detection of NO in IBD and may promote inflammatory stool examination simultaneously.
炎症性肠病（IBD）是一种常见的胃肠道炎症性疾病，影响着全世界大量的人群，且发病率逐年增加。尽管 IBD 的病因尚未完全阐明，但已知与 NO 产生的上调密切相关。在此，我们首次报道了一种供体-受体-供体（D-A-D）型近红外（NIR）荧光探针，通过利用增强的分子内电荷转移机制，实时检测 IBD 中的 NO。 表现出良好的水溶性、高光稳定性以及在 700nm 和 750/800nm 处的优异近红外吸收和发射。此外，它能够在活细胞的溶酶体中灵敏且特异性地检测外源性和内源性 NO。值得注意的是， 能够非侵入性地可视化脂多糖诱导的 IBD 小鼠模型中 30 小时的 NO 生成，在 IBD 小鼠的肠道和粪便中的近红外荧光强度比正常小鼠高 2-3 倍。这项工作表明 是一种有前途的诊断试剂，可用于实时检测 IBD 中的 NO，并可能同时促进炎症性粪便检查。
