School of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, China.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
J Nanosci Nanotechnol. 2021 Sep 1;21(9):4573-4578. doi: 10.1166/jnn.2021.19348.
In this investigation, a sensitive sensor for ochratoxin A detection was designed based on graphene oxide (GO) and DNase I for amplified fluorescence signal. The outcomes of this investigation indicated that this exploratory platform exhibited efficient sensitivity and good selectivity for ochratoxin A when existence of other toxins. The proposed sensor displayed a linear response over a range of 100-1000 nM (² = 0.986) with a low detection limit of 38.2 nM (S/N = 3) and showed remarkable convenience. In addition, the constructed sensor was examined by testing real food samples spiked with different concentrations of ochratoxin A. Results demonstrated that the constructed sensor provided an effective method for ochratoxin A evaluate in complicated food sample analyses.
本研究基于氧化石墨烯（GO）和 DNA 酶 I 设计了一种用于放大荧光信号的灵敏的赭曲霉毒素 A 检测传感器。研究结果表明，该探索性平台在存在其他毒素时对赭曲霉毒素 A 表现出高效的灵敏度和良好的选择性。该传感器在 100-1000 nM 的范围内呈现出线性响应（² = 0.986），检测限低至 38.2 nM（S/N = 3），具有显著的便利性。此外，通过测试不同浓度赭曲霉毒素 A 掺杂的实际食品样品对所构建的传感器进行了评估。结果表明，该构建的传感器为复杂食品样品分析中评估赭曲霉毒素 A 提供了一种有效的方法。
