Woo N D, Persaud T V
Department of Anatomy, University of Manitoba, Winnipeg, Canada.
Acta Anat (Basel). 1988;131(2):122-6. doi: 10.1159/000146498.
Pregnant Sprague-Dawley rats were treated from day 9 through 12 of gestation with either ethanol (12.5% v/v in physiological saline, 0.015 ml/g, i.p.) and nicotine (16.7 mg/kg, s.c.), alone or in combination. Corresponding groups of animals received physiological saline in a similar manner and these served as controls. Embryos were recovered on gestational day 12 and were quantitatively assessed on the basis of 16 recognizable developmental endpoints (morphological scores). The head and the crown-rump lengths of the embryos were also measured. Embryos exposed to nicotine and to ethanol-nicotine were significantly affected in their development whereas those exposed to ethanol alone were spared.
妊娠第9天至12天期间，对怀孕的斯普拉格-道利大鼠分别单独或联合给予乙醇（生理盐水中体积分数为12.5%，0.015 ml/g，腹腔注射）和尼古丁（16.7 mg/kg，皮下注射）。相应的动物组以类似方式接受生理盐水，作为对照。在妊娠第12天取出胚胎，并根据16个可识别的发育终点（形态学评分）进行定量评估。还测量了胚胎的头部和顶臀长度。暴露于尼古丁和乙醇-尼古丁的胚胎发育受到显著影响，而仅暴露于乙醇的胚胎则未受影响。
