Seyoum G, Persaud T V
Department of Anatomy, University of Manitoba, Winnipeg, Canada.
Exp Toxicol Pathol. 1995 Jan;47(1):75-9. doi: 10.1016/S0940-2993(11)80290-3.
Zinc is a cofactor for alcohol dehydrogenase, the ethanol metabolizing enzyme. Ethanol-induced zinc deficiency could decrease ethanol metabolism, resulting in an increase in circulating and tissue ethanol levels. This may cause retardation in embryonic growth and development. The influence of zinc supplementation on ethanol-induced embryopathy was studied by the simultaneous administration of ethanol and zinc to pregnant SD rats from gestational day 6 through 12. Ethanol was given in the form of a liquid diet and zinc was administered intraperitoneally. The ethanol group received the liquid ethanol diet, the ethanol+zinc group received the ethanol diet and zinc and the pair-fed control group was given an isocaloric control diet. Embryos were explanted from all groups on day 12 of gestation. Embryos of animals treated with ethanol alone exhibited a significantly higher rate of resorption and retarded embryonic growth and development compared to the pair-fed control group. The embryonic protein content, crown-rump length, the number of somites and embryonic morphological score were significantly reduced in the ethanol-treated group. In addition, serum zinc concentration also was lower. Compared to embryos from ethanol-treated animals, embryos from ethanol+zinc treated animals showed a significantly higher number of somites; cardiac development was more advanced and embryonic protein content was higher. These observations suggest that zinc supplementation of ethanol-treated pregnant rats may have some protective influence against the embryopathic effects of ethanol.
锌是乙醇代谢酶乙醇脱氢酶的一种辅助因子。乙醇诱导的锌缺乏会降低乙醇代谢，导致循环和组织中乙醇水平升高。这可能会导致胚胎生长发育迟缓。通过从妊娠第6天至12天同时给怀孕的SD大鼠喂食乙醇和锌，研究了补充锌对乙醇诱导的胚胎病的影响。乙醇以液体饲料的形式给予，锌通过腹腔注射给药。乙醇组接受液体乙醇饲料，乙醇+锌组接受乙醇饲料和锌，配对喂养对照组给予等热量对照饲料。在妊娠第12天从所有组中取出胚胎。与配对喂养对照组相比，单独用乙醇处理的动物的胚胎吸收发生率显著更高，胚胎生长发育迟缓。乙醇处理组的胚胎蛋白质含量、顶臀长度、体节数量和胚胎形态评分显著降低。此外，血清锌浓度也较低。与乙醇处理动物的胚胎相比，乙醇+锌处理动物的胚胎体节数量显著更多；心脏发育更成熟，胚胎蛋白质含量更高。这些观察结果表明，给乙醇处理的怀孕大鼠补充锌可能对乙醇的胚胎病效应有一定的保护作用。
