Huertas-Rosales Óscar, Romero Manuel, Chan Kok-Gan, Hong Kar-Wai, Cámara Miguel, Heeb Stephan, Barrientos-Moreno Laura, Molina-Henares María Antonia, Travieso María L, Ramos-González María Isabel, Espinosa-Urgel Manuel
Department of Environmental Protection, Estación Experimental del Zaidín, CSIC, Granada, Spain.
National Biofilms Innovation Centre, Biodiscovery Institute and School of Life Sciences, University of Nottingham, Nottingham, United Kingdom.
Front Mol Biosci. 2021 Feb 22;8:624061. doi: 10.3389/fmolb.2021.624061. eCollection 2021.
Post-transcriptional regulation is an important step in the control of bacterial gene expression in response to environmental and cellular signals. KT2440 harbors three known members of the CsrA/RsmA family of post-transcriptional regulators: RsmA, RsmE and RsmI. We have carried out a global analysis to identify RNA sequences bound by each of these proteins. Affinity purification and sequencing of RNA molecules associated with Rsm proteins were used to discover direct binding targets, corresponding to 437 unique RNA molecules, 75 of them being common to the three proteins. Relevant targets include genes encoding proteins involved in signal transduction and regulation, metabolism, transport and secretion, stress responses, and the turnover of the intracellular second messenger c-di-GMP. To our knowledge, this is the first combined global analysis in a bacterium harboring three Rsm homologs. It offers a broad overview of the network of processes subjected to this type of regulation and opens the way to define what are the sequence and structure determinants that define common or differential recognition of specific RNA molecules by these proteins.
转录后调控是细菌响应环境和细胞信号进行基因表达控制的重要步骤。KT2440含有转录后调控因子CsrA/RsmA家族的三个已知成员：RsmA、RsmE和RsmI。我们进行了一项全局分析，以鉴定与这些蛋白质各自结合的RNA序列。通过对与Rsm蛋白相关的RNA分子进行亲和纯化和测序，来发现直接结合靶点，共对应437个独特的RNA分子，其中75个是这三种蛋白质共有的。相关靶点包括编码参与信号转导与调控、代谢、运输与分泌、应激反应以及细胞内第二信使c-di-GMP周转的蛋白质的基因。据我们所知，这是在含有三个Rsm同源物的细菌中首次进行的联合全局分析。它提供了受此类调控的过程网络的广泛概述，并为确定定义这些蛋白质对特定RNA分子的共同或差异识别的序列和结构决定因素开辟了道路。
