Glasgow B J, Brown H H, Zargoza A M, Foos R Y
Jules Stein Eye Institute, Los Angeles, CA 90024.
Am J Ophthalmol. 1988 May 15;105(5):538-46. doi: 10.1016/0002-9394(88)90248-6.
Twenty-two fine needle (30 gauge) aspirations were performed in eyes enucleated for the clinical diagnosis of melanoma. Cytologic preparations were evaluated for adequacy of material, and needle tracts were evaluated for tumor implantation. A scleral marking method was used to identify all needle tracts. The number of tumor cells in tracts of direct transscleral aspirates was compared to those in tracts of indirect aspirates that traversed the anterior chamber or vitreous. Cellular material obtained with 30-gauge needles was sufficient for the diagnosis of malignant melanoma in all but one case. While 14 of 21 (67%) of all fine needle aspiration tracts and eight of 15 (53%) of indirect tracts contained tumor cells, the number of tumor cells was less than that associated with tumor growth in experimental models. Indirect aspirate tracts contained significantly fewer cells than tracts of direct aspirates (P less than .001).
对22只因临床诊断黑色素瘤而摘除的眼球进行了细针（30号）抽吸。评估细胞涂片制备的取材是否充分，并评估针道有无肿瘤种植。采用巩膜标记法识别所有针道。比较经巩膜直接抽吸针道中的肿瘤细胞数量与经前房或玻璃体的间接抽吸针道中的肿瘤细胞数量。除1例之外，用30号针获取的细胞材料足以诊断恶性黑色素瘤。在所有细针抽吸针道中，21个中有14个（67%）以及在15个间接针道中有8个（53%）含有肿瘤细胞，但肿瘤细胞数量少于实验模型中与肿瘤生长相关的数量。间接抽吸针道中的细胞数量明显少于直接抽吸针道中的细胞数量（P<0.001）。
