Brotherton J
Department of Gynaecological Endocrinology, Klinikum Steglitz of the Free University Berlin, Germany.
Andrologia. 1988 Jan-Feb;20(1):33-43. doi: 10.1111/j.1439-0272.1988.tb02358.x.
For 25 consecutive human semen samples, a comparison was made of sperm count and sperm motility values obtained by routine manual methods and by using a machine that measures these functions by analysing the deflection of an impinging laser beam (Lazymot machine). Sperm counts in undiluted semen were approximately 5 times higher with the laser machine. As sperm counts increased to about 300 million/ml the counts obtained by the two methods converged as the chance of the beam hitting a spermatozoon and not another type of particle increased. In semen diluted 1 + 4 with Baker's solution, the uncorrected laser count agreed well with the sperm count obtained using a haemocytometer. Multiplication of the laser count by 5 did not reach the same count as that measured in the undiluted sample, showing that the dilution had dissolved some of the smaller particles. It was recommended to measure laser percentage motility in undiluted semen but the values obtained bore no relationship to those obtained using a haemocytometer and neither did the values obtained for laser percentage sperm with progressive motility. The mean laser velocity of the total motility was 23-64 micron/sec and for the progressive particles was 48-84 micron/sec, values which were much faster that the acceptably normal values of 8-30 micron/sec found for selected progressively motile spermatozoa timed with a stopwatch. The laser machine detected an increase in counts and the presence of residual motility after cytoplasm had been stripped away from the spermatozoa with a saponin reagent. The laser machine was unable to detect any increase in speed on increasing the temperature to 37 degrees C. It was concluded that the Lazymot machine as presently designed is not useful in the andrological laboratory for routine counting and motility determinations, mainly due to the absence of a size discriminator against the multitude of small particles that are present in human semen.
对连续25份人类精液样本，比较了通过常规手工方法以及使用一种通过分析入射激光束的偏转来测量这些功能的机器（Lazymot机器）获得的精子计数和精子活力值。未稀释精液中的精子计数，激光机器测得的约为手工方法的5倍。随着精子计数增加到约3亿/ml，两种方法获得的计数趋于一致，因为光束击中精子而非其他类型颗粒的几率增加。在用贝克溶液1 + 4稀释的精液中，未经校正的激光计数与使用血细胞计数器获得的精子计数吻合良好。将激光计数乘以5并未达到未稀释样本中测得的计数，表明稀释溶解了一些较小的颗粒。建议在未稀释精液中测量激光活力百分比，但获得的值与使用血细胞计数器获得的值无关，具有进行性运动的激光精子百分比的值也无关。总活力的平均激光速度为23 - 64微米/秒，进行性颗粒的为48 - 84微米/秒，这些值比用秒表计时的选定进行性运动精子的可接受正常范围8 - 30微米/秒快得多。在用皂素试剂从精子中去除细胞质后，激光机器检测到计数增加以及残余活力的存在。将温度升至37摄氏度时，激光机器未能检测到速度有任何增加。得出的结论是，目前设计的Lazymot机器在男科学实验室中对于常规计数和活力测定并无用处，主要原因是缺乏针对人类精液中存在的大量小颗粒的大小鉴别器。
