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[在常规男科学实验室检测中通过激光多普勒光谱法测量精子活力]

[Measurement of sperm motility by Laser-Doppler-spectroscopy in routine andrology lab tests].

作者信息

Pusch H H

出版信息

Andrologia. 1985 May-Jun;17(3):217-23.

PMID:4025839
Abstract

Sperm motility of 177 ejaculates from the andrology laboratory, University Clinic for Gynecology and Obstetrics, Graz/Austria, was evaluated in a comparative study. Motility was measured by Laser-Doppler-Spectroscopy (Lazymot) and by means of conventional microscopic estimations. For the first time results gained with Lazymot have been associated to different spermiogram diagnoses. In addition to measurements of percentages of total sperm motility, evaluations of mean sperm motility in micron/sec. have been performed. Correlation of results was good for both methods, besides the problem groups of ejaculates with sperm densities below ten millions of spermatozoa per milliliter. Motility characteristics of spermatozoa in an in vitro-Fertilization-Program have been subject to objective measurements with Lazymot for the first time. An enhancement of total motility (16%) and of mean spermatozoal velocity (36%) could be observed in the fertilization media after capacitation. Laser-Doppler-Spectroscopy represents a reliable and fast method for motility determinations in human ejaculate. It is suited for research purposes as well as for andrologic routine work. The operation of the Lazymot is considered to be unproblematic.

摘要

在一项比较研究中,对奥地利格拉茨大学妇产科男科实验室的177份精液样本的精子活力进行了评估。精子活力通过激光多普勒光谱法(Lazymot)和传统显微镜评估法进行测量。首次将Lazymot获得的结果与不同的精子图谱诊断相关联。除了测量总精子活力百分比外,还对平均精子活力(微米/秒)进行了评估。除了每毫升精子密度低于1000万个精子的精液问题组外,两种方法的结果相关性良好。首次使用Lazymot对体外受精程序中精子的活力特征进行了客观测量。在获能后的受精培养基中,可以观察到总活力提高了16%,平均精子速度提高了36%。激光多普勒光谱法是一种可靠、快速的人类精液活力测定方法。它适用于研究目的以及男科日常工作。Lazymot的操作被认为没有问题。

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