佛波醇肉豆蔻酸酯乙酸盐和伴刀豆球蛋白A对人外周血淋巴细胞糖酵解酶的影响。

Effect of phorbol myristate acetate and concanavalin A on the glycolytic enzymes of human peripheral lymphocytes.

作者信息

Marjanovic S, Wielburski A, Nelson B D

机构信息

Department of Biochemistry, Arrhenius Laboratory, University of Stockholm, Sweden.

出版信息

Biochim Biophys Acta. 1988 Jun 8;970(1):1-6. doi: 10.1016/0167-4889(88)90215-7.

DOI:10.1016/0167-4889(88)90215-7

PMID:3370225

Abstract

The effects of Concanavalin A and the tumor promoting agent, phorbol 12-myristate 13-acetate (PMA), on glycolytic enzymes in human peripheral lymphocytes have been studied. A combination of Concanavalin A plus PMA stimulates DNA and protein synthesis to a significantly greater extent than when each are added individually. PMA and concanavalin A together, but not individually, also increase the levels of the activity of the glycolytic enzymes in peripheral lymphocytes treated for 48 h. The increase in hexokinase activity induced by PMA plus concanavalin A appeared to be due to the expression of the isoenzyme form, hexokinase II. The results suggest that the expression of glycolytic enzymes in stimulated lymphocytes is a late event (perhaps associated with the S phase) which is regulated by a cellular signal system controlled by the combined action of PMA plus concanavalin A.

摘要

已对伴刀豆球蛋白A和肿瘤促进剂佛波酯（phorbol 12 - 肉豆蔻酸酯13 - 乙酸酯，PMA）对人外周血淋巴细胞中糖酵解酶的影响进行了研究。伴刀豆球蛋白A与PMA联合使用时，刺激DNA和蛋白质合成的程度明显大于单独添加时。PMA和伴刀豆球蛋白A共同作用（而非单独作用），也会增加处理48小时的外周血淋巴细胞中糖酵解酶的活性水平。PMA加伴刀豆球蛋白A诱导的己糖激酶活性增加似乎是由于同工酶形式——己糖激酶II的表达。结果表明，受刺激淋巴细胞中糖酵解酶的表达是一个晚期事件（可能与S期相关），该事件由PMA加伴刀豆球蛋白A的联合作用所控制的细胞信号系统调节。