佛波醇肉豆蔻酸酯乙酸酯：一种对T淋巴细胞亚群具有选择性的促有丝分裂原。
Phorbol myristate acetate: a mitogen selective for a T-lymphocyte subpopulation.
作者信息
Touraine J L, Hadden J W, Touraine F, Hadden E M, Estensen R, Good R A
出版信息
J Exp Med. 1977 Feb 1;145(2):460-5. doi: 10.1084/jem.145.2.460.
Abstract
Phorbol myristate acetate (pma) is a potent mitogen for human peripheral blood lymphocytes (PBL) comparable to phytohemagglutinin (PHA) in potency. Inactivation of PHA-responsive lymphocytes by 5'-bromodeoxyuridine and light treatment left the PMA response intact and nice versa. Experiments separating lymphocytes by rosetting with sheep erythrocytes (SRBC) demonstrated that the PMA-responsive lymphocytes segregate with those that have a high affinity for SRBC to a greater than PHA- or concanavalin A (Con A)-responsive cells. These results indicate that a PMA-responsive population in human peripheral blood resides within the T-lymphocyte population and appears to have a high affinity for SRBC and to be distinct from that responding to PHA and Con A. PMA may be useful clinically to assay the size and function of the high affinity or "active" rosette population.
摘要
佛波醇肉豆蔻酸酯乙酸盐（PMA）是一种对人外周血淋巴细胞（PBL）有强大作用的丝裂原，其效力与植物血凝素（PHA）相当。用5'-溴脱氧尿苷和光照处理使对PHA有反应的淋巴细胞失活后，PMA反应仍保持完整，反之亦然。通过与绵羊红细胞（SRBC）进行玫瑰花结形成实验来分离淋巴细胞，结果表明，对PMA有反应的淋巴细胞与那些对SRBC具有高亲和力的淋巴细胞聚集在一起，其程度大于对PHA或刀豆球蛋白A（Con A）有反应的细胞。这些结果表明，人外周血中对PMA有反应的细胞群体存在于T淋巴细胞群体中，似乎对SRBC具有高亲和力，并且与对PHA和Con A有反应的细胞群体不同。PMA在临床上可能有助于检测高亲和力或“活性”玫瑰花结细胞群体的大小和功能。
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