Pshenichkin S F, Zozulia A A, Kizim E A, Dmitriev A D
Biokhimiia. 1988 Feb;53(2):309-16.
Immunoreactive alpha-, beta-, gamma-endorphins and beta-lipotropin were detected in perfused calf thymus extracts at the following concentrations (fmol/mg) tissue, M +/- m): 1.32 +/- 0.08, 1.53 +/- 0.45, 0.0186 +/- 0.0022 and 0.741 +/- 0.157, respectively. It was demonstrated for all ligands tested that the synthetic peptide and increasing amounts of the extract cause a similar displacement of the corresponding 125I-peptide from its complex with specific antiserum. Using the immunoblotting technique with a highly specific antiserum to bovine beta-lipotropin, the extracts of calf thymus, rat thymocytes and bovine hypophysis were found to contain two polypeptides with Mr of 32 and 14 kD, whose mobility corresponds to that of proopiomelanocortin and beta-lipotropin.
在灌注的小牛胸腺提取物中检测到免疫反应性α-、β-、γ-内啡肽和β-促脂素，其浓度（fmol/mg组织，M±m）如下：分别为1.32±0.08、1.53±0.45、0.0186±0.0022和0.741±0.157。对于所有测试的配体，均证明合成肽和提取物量的增加会导致相应的125I肽从其与特异性抗血清的复合物中产生类似的位移。使用针对牛β-促脂素的高度特异性抗血清的免疫印迹技术，发现小牛胸腺、大鼠胸腺细胞和牛垂体的提取物含有两种分子量分别为32kD和14kD的多肽，其迁移率与阿黑皮素原和β-促脂素的迁移率一致。
