Tennov A V, Dmitriev A D, Kizim E A, Ustinova E E
Biull Eksp Biol Med. 1985 Aug;100(8):181-4.
Immunoreactive alpha-, beta- and gamma-endorphins and beta-lipotropin--C-terminal peptide fragments of pro-opiomelanocortin (POMC)--were discovered and measured by RIA in the bovine adrenal medulla and cortex. These peptides were also discovered in perfusates of the adrenal gland. POMC proper and some intermediate forms of its processing not differing in electrophoretic mobility from the respective molecular forms of hypophyseal POMC were identified in the medulla and cortex of the adrenals by the immunoblotting technique with the use of antiserum to beta-lipoprotein. It is concluded that POMC gene is expressed in the adrenal medulla and cortex and that as a result of POMC processing a noticeable amount of its peptide fragments is formed and secreted in adrenal cells. The authors thus suggest the presence of existence of the pituitary-unrelated mechanisms of adrenal function control with participation of POMC peptides synthesized in the adrenals.
通过放射免疫分析法（RIA）在牛肾上腺髓质和皮质中发现并测定了免疫反应性α-、β-和γ-内啡肽以及β-促脂素——促阿片-黑素细胞皮质素原（POMC）的C末端肽片段。在肾上腺灌流液中也发现了这些肽。利用抗β-脂蛋白抗血清通过免疫印迹技术在肾上腺髓质和皮质中鉴定出了POMC本身及其一些加工中间形式，这些中间形式在电泳迁移率上与垂体POMC的相应分子形式没有差异。得出的结论是，POMC基因在肾上腺髓质和皮质中表达，并且由于POMC的加工，其大量肽片段在肾上腺细胞中形成并分泌。因此，作者认为存在与垂体无关的肾上腺功能控制机制，肾上腺中合成的POMC肽参与其中。
