Laboratory of Gerontology, Department of Zoology, Bangalore University, Bangalore, India.
Department of Life Sciences, Bangalore University, Bangalore, India.
Arch Physiol Biochem. 2023 Oct;129(5):1045-1057. doi: 10.1080/13813455.2021.1894452. Epub 2021 Mar 11.
This study aimed to elucidate the effects of grape seed proanthocyanidin extract (GSPE) on oxidative stress (OS), antioxidant enzymes, free radicals and cytokines in the pancreas of T1DM rats.
Two-month-old Wistar rats were assigned to the control (CON), CON + GSPE (CON + PA), diabetics (STZ, 60 mg/kg b.w.), diabetes + GSPE (STZ + PA), diabetes + insulin (STZ + INS, 3 U/day) and diabetics + GSPE and INS (STZ + INS + PA) groups. GSPE (75 mg/kg b.w.) was administered daily either alone or with INS for 8 weeks.
Glutathione was lowest in diabetics while it increased in the STZ + INS + PA ( < .001) group, similar to catalase activity ( < .05). Hydrogen peroxide, superoxide and lipid peroxidation increased with iNOS, TNF-α and IL-1β in the diabetic pancreases, while GSPE decreased ( < .001). Further, reduced β-cells/islet number was improved in diabetics ( < .001) with treatment.
This study suggests that GSPE with INS is effective in minimising OS and pancreatic degeneration in T1DM rats.
本研究旨在阐明葡萄籽原花青素提取物（GSPE）对 T1DM 大鼠胰腺氧化应激（OS）、抗氧化酶、自由基和细胞因子的影响。
将 2 月龄 Wistar 大鼠分为对照组（CON）、CON+GSPE（CON+PA）、糖尿病组（STZ，60mg/kg b.w.）、糖尿病+GSPE 组（STZ+PA）、糖尿病+胰岛素组（STZ+INS，3U/天）和糖尿病+GSPE 和胰岛素组（STZ+INS+PA）。GSPE（75mg/kg b.w.）单独或与 INS 一起每天给药 8 周。
糖尿病大鼠谷胱甘肽含量最低，而 STZ+INS+PA 组则增加（ < .001），与过氧化氢酶活性相似（ < .05）。超氧化物歧化酶、过氧化氢、脂质过氧化物和诱导型一氧化氮合酶、肿瘤坏死因子-α和白细胞介素-1β在糖尿病胰腺中增加，而 GSPE 则减少（ < .001）。此外，糖尿病大鼠的β细胞/胰岛数量减少（ < .001），经治疗后得到改善。
本研究表明，GSPE 与 INS 联合使用可有效减轻 T1DM 大鼠的 OS 和胰腺变性。
