Norwich Medical School, University of East Anglia, Norwich, UK.
Methods Mol Biol. 2021;2291:273-283. doi: 10.1007/978-1-0716-1339-9_12.
The environment in the human intestine is low in oxygen. This affects virulence gene expression of enteropathogens including Shiga toxin-producing E. coli (STEC) and enables mucosal colonization by oxygen-sensitive commensal bacteria. To simulate the oxygen-restricted milieu at the intestinal epithelium, we have developed a vertical diffusion chamber model (VDC) which allows infection of polarized human intestinal epithelia under microaerobic conditions. In this chapter, we present a detailed protocol for performing STEC infections in the VDC system and subsequent analysis of STEC pathogenesis.
人类肠道内的环境含氧量低。这种低氧环境会影响包括产志贺毒素大肠杆菌（STEC）在内的肠道病原体的毒力基因表达，并使对氧气敏感的共生菌在黏膜定植。为了模拟肠道上皮细胞的缺氧环境，我们开发了一种垂直扩散室模型（VDC），该模型允许在微需氧条件下感染极化的人肠道上皮细胞。在本章中，我们将详细介绍在 VDC 系统中进行 STEC 感染以及随后分析 STEC 发病机制的方法。
