Division of Gastroenterology, Department of Internal Medicine, University of New Mexico Health Sciences Center, University of New Mexico School of Medicine, Albuquerque, NM, USA.
Department of Microbiology & Immunology, University of Maryland School of Medicine, Baltimore, MD, USA.
Methods Mol Biol. 2021;2291:285-296. doi: 10.1007/978-1-0716-1339-9_13.
Human intestinal organoid cultures established from crypt-derived stem cells truly revolutionized our approach to study intestinal epithelial physiology and pathologies as they can be propagated indefinitely and preserve the genetic signature of the donor and the gut segment specificity in culture. Here we describe human stem cell-derived colonoid monolayers as a reliable and reproducible model to study Shiga toxin-producing Escherichia coli (STEC) infection and STEC-caused pathologies of the whole colonic epithelium comprising a mixture of colonocytes, goblet, enteroendocrine, and other rare cells present in human colonic epithelial tissue.
从隐窝衍生的干细胞建立的人类肠道类器官培养物真正改变了我们研究肠道上皮生理学和病理学的方法，因为它们可以无限期繁殖，并在培养中保留供体的遗传特征和肠道节段特异性。在这里，我们将人类干细胞衍生的结肠类器官单层描述为研究产志贺毒素大肠杆菌（STEC）感染和 STEC 引起的整个结肠上皮病变的可靠和可重复的模型，该模型包含混合了存在于人结肠上皮组织中的结肠细胞、杯状细胞、肠内分泌细胞和其他稀有细胞。
