Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan.
Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
J Med Virol. 2021 Aug;93(8):4865-4874. doi: 10.1002/jmv.26934. Epub 2021 Mar 25.
Sapovirus (SaV) is one of the pathogens related to acute gastroenteritis (AGE) in adults and children worldwide. This study reported the diversity of SaV genotypes in children with AGE in Japan from July 2014 to June 2017. Of a total of 2259 stool samples tested by using reverse transcription-PCR method and further analyzed by nucleotide sequencing, 114 (5.0%) were positive for SaV and GI.1 (83.3%) was the most predominant genotype, followed by GII.1, GIV.1, GI.2, GI.3, and GII.3 genotypes. Monthly distribution analysis demonstrated two epidemic peaks from July to December 2015 and February to May 2017. However, no detection peak was observed in 2014 and 2016. Phylogenetic analysis of the complete VP1 nucleotide sequences of these GI.1 strains revealed two major clusters of GI.1 and each of which contained GI.1 strains of both 2015 and 2017. This study suggests that the continuous surveillance of SaV is needed to monitor high genetic diversity in Japanese children with AGE.
肠病毒（SaV）是全球范围内导致成人和儿童急性肠胃炎（AGE）的病原体之一。本研究报告了 2014 年 7 月至 2017 年 6 月日本 AGE 患儿中 SaV 基因型的多样性。在总共 2259 份通过逆转录-PCR 方法检测的粪便样本中，有 114 份（5.0%）对 SaV 和 GI.1 呈阳性，其中 GI.1 基因型最为主要，其次是 GII.1、GIV.1、GI.2、GI.3 和 GII.3 基因型。月度分布分析显示，2015 年 7 月至 12 月和 2017 年 2 月至 5 月有两个流行高峰。然而，2014 年和 2016 年没有检测到高峰。对这些 GI.1 株的完整 VP1 核苷酸序列的系统进化分析显示，GI.1 存在两个主要聚类，每个聚类均包含 2015 年和 2017 年的 GI.1 株。本研究表明，需要对 SaV 进行持续监测，以监测日本 AGE 患儿中 SaV 的高遗传多样性。
