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转录组分析揭示了辣椒（Capsicum annuum L.）对野油菜黄单胞菌 pv.vesicatoria 的防御相关基因和途径。

Transcriptome analysis reveals defense-related genes and pathways against Xanthomonas campestris pv. vesicatoria in pepper (Capsicum annuum L.).

机构信息

Hubei Key Laboratory of Vegetable Germplasm Enhancement and Genetic Improvement, Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan, Hubei, China.

Kasetsart University, Bangkok, Thailand.

出版信息

PLoS One. 2021 Mar 11;16(3):e0240279. doi: 10.1371/journal.pone.0240279. eCollection 2021.

DOI:10.1371/journal.pone.0240279
PMID:33705404
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7951875/
Abstract

Bacterial spot (BS), incited by Xanthomonas campestris pv. vesicatoria (Xcv), is one of the most serious diseases of pepper. For a comparative analysis of defense responses to Xcv infection, we performed a transcriptomic analysis of a susceptible cultivar, ECW, and a resistant cultivar, VI037601, using the HiSeqTM 2500 sequencing platform. Approximately 120.23 G clean bases were generated from 18 libraries. From the libraries generated, a total of 38,269 expressed genes containing 11,714 novel genes and 11,232 differentially expressed genes (DEGs) were identified. Functional enrichment analysis revealed that the most noticeable pathways were plant-pathogen interaction, MAPK signaling pathway-plant, plant hormone signal transduction and secondary metabolisms. 1,599 potentially defense-related genes linked to pattern recognition receptors (PRRs), mitogen-activated protein kinase (MAPK), calcium signaling, and transcription factors may regulate pepper resistance to Xcv. Moreover, after Xcv inoculation, 364 DEGs differentially expressed only in VI037601 and 852 genes in both ECW and VI037601. Many of those genes were classified as NBS-LRR genes, oxidoreductase gene, WRKY and NAC transcription factors, and they were mainly involved in metabolic process, response to stimulus and biological regulation pathways. Quantitative RT-PCR of sixteen selected DEGs further validated the RNA-seq differential gene expression analysis. Our results will provide a valuable resource for understanding the molecular mechanisms of pepper resistance to Xcv infection and improving pepper resistance cultivars against Xcv.

摘要

细菌斑点病（BS）是由野油菜黄单胞菌辣椒致病变种（Xcv）引起的辣椒最严重的病害之一。为了比较分析对 Xcv 感染的防御反应，我们使用 HiSeqTM 2500 测序平台对感病品种 ECW 和抗病品种 VI037601 进行了转录组分析。从 18 个文库中产生了大约 120.23 G 的清洁碱基。从生成的文库中，共鉴定出 38269 个表达基因，其中包含 11714 个新基因和 11232 个差异表达基因（DEGs）。功能富集分析表明，最显著的途径是植物-病原体相互作用、MAPK 信号通路-植物、植物激素信号转导和次生代谢。与模式识别受体（PRRs）、丝裂原活化蛋白激酶（MAPK）、钙信号和转录因子相关的 1599 个潜在防御相关基因可能调节辣椒对 Xcv 的抗性。此外，在接种 Xcv 后，VI037601 中仅差异表达 364 个 DEG，而在 ECW 和 VI037601 中差异表达 852 个基因。其中许多基因被归类为 NBS-LRR 基因、氧化还原酶基因、WRKY 和 NAC 转录因子，它们主要参与代谢过程、对刺激的反应和生物调节途径。十六个选定的 DEG 的定量 RT-PCR 进一步验证了 RNA-seq 差异基因表达分析。我们的研究结果将为理解辣椒对 Xcv 感染的抗性分子机制以及提高辣椒对 Xcv 的抗性品种提供有价值的资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4492/7951875/c69914dd1c4c/pone.0240279.g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4492/7951875/97b4611308c3/pone.0240279.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4492/7951875/c69914dd1c4c/pone.0240279.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4492/7951875/800b8a093a45/pone.0240279.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4492/7951875/267523962e24/pone.0240279.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4492/7951875/b25ca6fd244f/pone.0240279.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4492/7951875/09f64cf39bd5/pone.0240279.g004.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4492/7951875/97b4611308c3/pone.0240279.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4492/7951875/c69914dd1c4c/pone.0240279.g007.jpg

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