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血清脂质谱及差异脂质与社区获得性肺炎严重程度的关系：一项初步研究。

Lipid profiles and differential lipids in serum related to severity of community-acquired pneumonia: A pilot study.

作者信息

Chen Li, Zheng Yali, Zhao Lili, Zhang Ying, Yin Lu, He Yukun, Ma Xinqian, Xu Yu, Gao Zhancheng

机构信息

Department of Respiratory & Critical Care Medicine, Peking University People's Hospital, Beijing, China.

Department of Respiratory, Critical Care & Sleep Medicine, Xiang'an Hospital of Xiamen University, Xiamen, China.

出版信息

PLoS One. 2021 Mar 11;16(3):e0245770. doi: 10.1371/journal.pone.0245770. eCollection 2021.

DOI:10.1371/journal.pone.0245770
PMID:33705428
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7951898/
Abstract

This study aimed to characterize the lipidomic responses to community-acquired pneumonia (CAP) and provide new insight into the underlying mechanisms of pathogenesis and potential avenues for diagnostic and therapeutic treatments. This study was performed from January 2017 to October 2018. Lipidomic profiles were generated using ultra high-performance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS) platform. Spearman's rank correlation test and multiple linear regression analysis were applied to explore the correlation between changes in the relative abundance of lipids and clinical parameters. Kaplan-Meier methods were used to build 30-day survival curves. From the UHPLC-MS/MS results, a total of 509 and 195 lipid species were detected in the positive and negative ionization mode respectively. Positive ionization covered six lipid classes (glycerol-phospholipids, glycerolipids, sphingolipids, sterol-lipids, prenol-lipids, and fatty acid), whilst negative ionization covered three (glycerol-phospholipids, sphingolipids, fatty acid). Four lipids were selected as targets: PC (16:0_18:1), PC (18:2_20:4), PC (36:4), and PC (38:6). The relative increase of the areas under the curves for all four lipids were superior to the pneumonia severity index and CURB-65 (confusion, urea, respiratory rate, blood pressure, and age ≥65 years old) for discriminating severe CAP from CAP. Decreasing relative levels of PC (18:2_20:4), PC (38:6), and PC (36:4) were negatively related to fraction of inspiration O2; Changes in the relative abundance of PC (16:0_18:1) and PC (18:2_20:4) had significantly linear relationship with procalcitonin. Patients with an elevated level of PC (16:0_18:1) had significantly longer duration of hospital stays. As the relative abundance of PC (18:2_20:4), PC (36:4), and PC (38:6) decreased, the length of hospitalization days and 30-day mortality rate increased significantly (all log-rank p<0.05). Therefore, using the UHPLC-MS/MS platform's serum lipidomic approach can help reveal changes in lipid abundance during CAP and establish lipid profiles related to disease severity.

摘要

本研究旨在描述社区获得性肺炎（CAP）的脂质组学反应，并为发病机制的潜在机制以及诊断和治疗的潜在途径提供新的见解。本研究于2017年1月至2018年10月进行。使用超高效液相色谱串联质谱（UHPLC-MS/MS）平台生成脂质组学图谱。应用Spearman等级相关检验和多元线性回归分析来探索脂质相对丰度变化与临床参数之间的相关性。采用Kaplan-Meier方法构建30天生存曲线。根据UHPLC-MS/MS结果，在正离子和负离子模式下分别共检测到509种和195种脂质。正离子模式涵盖六种脂质类别（甘油磷脂、甘油酯、鞘脂、甾醇脂质、异戊二烯脂质和脂肪酸），而负离子模式涵盖三种（甘油磷脂、鞘脂、脂肪酸）。选择四种脂质作为靶点：PC（16:0_18:1）、PC（18:2_20:4）、PC（36:4）和PC（38:6）。所有四种脂质曲线下面积的相对增加在区分重症CAP与CAP方面优于肺炎严重程度指数和CURB-65（意识模糊、尿素、呼吸频率、血压和年龄≥65岁）。PC（18:2_20:4）、PC（38:6）和PC（36:4）相对水平的降低与吸入氧分数呈负相关；PC（16:0_18:1）和PC（18:2_20:4）相对丰度的变化与降钙素原具有显著的线性关系。PC（16:0_18:1）水平升高的患者住院时间显著更长。随着PC（18:2_20:4）、PC（36:4）和PC（38:6）相对丰度的降低，住院天数和30天死亡率显著增加（所有对数秩检验p<0.05）。因此，使用UHPLC-MS/MS平台的血清脂质组学方法有助于揭示CAP期间脂质丰度的变化，并建立与疾病严重程度相关的脂质谱。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d9d/7951898/c4a88d6efc9a/pone.0245770.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d9d/7951898/218cb7b0aaa1/pone.0245770.g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d9d/7951898/218cb7b0aaa1/pone.0245770.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d9d/7951898/6096b0d983b8/pone.0245770.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d9d/7951898/0ab99ec67f99/pone.0245770.g003.jpg
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