Huang Jinfeng, Wang Qi, Qi Zhenhua, Zhou Shixiang, Zhou Meijuan, Wang Zhidong
Department of Radiation Medicine, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, People's Republic of China.
Department of Radiobiology, Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing, People's Republic of China.
Dose Response. 2020 Apr 23;18(2):1559325820914209. doi: 10.1177/1559325820914209. eCollection 2020 Apr-Jun.
Radiation biodosimeters are required urgently for fast and accurate evaluation of absorbed dose for irradiated individuals. Lipidomics has appeared as a credible technique for identification and quantification of lipid for researching biomarker of diseases. We performed a lipidomic profile on mice serum at time points of 6, 24, and 72 hours after 0, 2, 5.5, 7, and 8 Gy irradiation to select radiation-responsive lipids and conducted Kyoto Encyclopedia of Genes and Genome pathway enrichment analysis to recognize the pathways and network changes. Then, Pearson correlation analysis was performed to evaluate the feasibility of radiation-responsive lipids to estimate radiation dose. Seven radiation-responsive lipids including PC (18:2/18:2), PC (18:0/18:2), Lyso PC 18:1, PC (18:0/20:4), SM (D18:0/24:1), PC (16:0/18:1), and Lyso PC 18:2 were identified in which glycerophospholipid metabolism presented as the most significant pathway, and they all presented good linear correlation with the irradiated dose. This study identified 7 radiation-responsive lipids in mice serum and certificate their feasibility of dose estimation as biodosimeters.
迫切需要辐射生物剂量计来快速准确地评估受辐照个体的吸收剂量。脂质组学已成为一种可靠的技术，用于鉴定和定量脂质，以研究疾病的生物标志物。我们在0、2、5.5、7和8 Gy辐照后的6、24和72小时时间点对小鼠血清进行脂质组学分析，以选择辐射响应性脂质，并进行京都基因与基因组百科全书通路富集分析，以识别通路和网络变化。然后，进行Pearson相关分析，以评估辐射响应性脂质估计辐射剂量的可行性。鉴定出7种辐射响应性脂质，包括PC(18:2/18:2)、PC(18:0/18:2)、溶血磷脂酰胆碱18:1、PC(18:0/20:4)、SM(D18:0/24:1)、PC(16:0/18:1)和溶血磷脂酰胆碱18:2，其中甘油磷脂代谢是最显著的通路，它们与辐照剂量均呈现良好的线性相关性。本研究在小鼠血清中鉴定出7种辐射响应性脂质，并证实了它们作为生物剂量计进行剂量估计的可行性。
