枣籽给药对链脲佐菌素诱导的糖尿病大鼠焦虑和抑郁症状的影响：生化及行为学证据

Effects of date seeds administration on anxiety and depressive symptoms in streptozotocin-induced diabetic rats: biochemical and behavioral evidences.

作者信息

Bikri Samir, Aboussaleh Youssef, Berrani Assia, Louragli Ismail, Hafid Affaf, Chakib Soukaina, Ahami Ahmed

机构信息

Biology Department, Faculty of Sciences, Laboratory of Nutrition, Health and Environment, Ibn Tofail University, Kenitra, Morocco.

Biology Department, Laboratory of Nutrition, Health and Environment, Ibn Tofail University, Kenitra, Morocco.

出版信息

J Basic Clin Physiol Pharmacol. 2021 Mar 12;32(6):1031-1040. doi: 10.1515/jbcpp-2020-0225.

DOI:10.1515/jbcpp-2020-0225

PMID:33705613

Abstract

OBJECTIVES

Several epidemiological data indicate that chronic hyperglycemia is associated with behavioral changes such as anxiety and depressive symptoms. Date seeds, one of the most potent products with potential antioxidant activities and possess many benefits against hyperglycemia and its complication. The aim of the current study was to explore the potential effect of date seeds extract on biochemical and behavioral changes (anxiety and depression) in streptozotocin (STZ)-diabetic rats.

METHODS

Rats were divided into four groups as follows: normal control, diabetic control, diabetic treated with the lyophilized aqueous extract of the date seed (2,000 mg/kg) (LAE-DS) and diabetics treated with insulin (4 UI/day). Experimental diabetes was induced by a single intraperitoneal injection of STZ (60 mg/kg). After 24 days treatment period, anxiety and depressive behaviors were evaluated using four behavioral tests. After sacrifice, blood samples were collected to evaluate lipid parameters. In addition, rat organs (kidney, liver and brain) were dissected out in order to estimate lipid peroxidation levels as oxidative stress marker.

RESULTS

Oral administration of the lyophilized aqueous extract of date seeds and insulin injection for 30 days significantly decreased blood glucose levels in STZ-diabetic rats and protected them against undesirable changes in lipid parameters, including cholesterol, triglycerides, LDL cholesterol, VLDL cholesterol and atherosclerosis index. Compared to untreated diabetic rat, a significant decrease in lipid peroxidation levels in kidney, liver and brain (Hippocampus and prefrontal cortex) were observed after treatment with insulin or LAE-DS in diabetic rats. Furthermore, insulin and LAE-DS administration prevented anxiety-related behaviors in STZ-diabetic rats.

CONCLUSIONS

Therefore, it would be possible to combine this extract with insulin and use it as an antioxidant supplement for type 1 diabetic patients.

摘要

目的

多项流行病学数据表明，慢性高血糖与焦虑和抑郁症状等行为变化有关。海枣种子是具有潜在抗氧化活性的最有效产品之一，对高血糖及其并发症有诸多益处。本研究的目的是探讨海枣种子提取物对链脲佐菌素（STZ）诱导的糖尿病大鼠生化和行为变化（焦虑和抑郁）的潜在影响。

方法

将大鼠分为四组：正常对照组、糖尿病对照组、用海枣种子冻干水提取物（2000毫克/千克）治疗的糖尿病组（LAE-DS）和用胰岛素（4单位/天）治疗的糖尿病组。通过单次腹腔注射STZ（60毫克/千克）诱导实验性糖尿病。治疗24天后，使用四项行为测试评估焦虑和抑郁行为。处死后，采集血样评估血脂参数。此外，解剖大鼠器官（肾脏、肝脏和大脑）以估计脂质过氧化水平作为氧化应激标志物。

结果

口服海枣种子冻干水提取物和注射胰岛素30天可显著降低STZ糖尿病大鼠的血糖水平，并保护它们免受血脂参数的不良变化影响，包括胆固醇、甘油三酯、低密度脂蛋白胆固醇、极低密度脂蛋白胆固醇和动脉粥样硬化指数。与未治疗的糖尿病大鼠相比，糖尿病大鼠用胰岛素或LAE-DS治疗后，肾脏、肝脏和大脑（海马体和前额叶皮层）的脂质过氧化水平显著降低。此外，胰岛素和LAE-DS给药可预防STZ糖尿病大鼠的焦虑相关行为。