Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.
Experimental Obesity and Diabetes Research Lab, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.
Vet Med Sci. 2021 Mar;7(2):500-511. doi: 10.1002/vms3.389. Epub 2020 Dec 5.
This study aimed to assess antihyperlipidemic, cardiac and antioxidant effects as well as mode of actions of Musa paradisiaca (M. paradisiaca) leaf and fruit peel hydroethanolic extracts in nicotinamide (NA)/streptozotocin (STZ)-induced diabetic rats. Experimental diabetes mellitus was induced by a single intraperitoneal injection of STZ (60 mg/kg body weight), 15 min after intraperitoneal injection of NA (120 mg/kg body weight). NA/STZ-induced diabetic rats were orally supplemented with M. paradisiaca leaf and fruit peel hydroethanolic extracts in a dose of 100 mg/kg body weight/day for 28 days. The treatment of NA/STZ-induced diabetic rats with M. paradisiaca leaf and fruit peel extracts significantly decreased the elevated fasting and post-prandial serum glucose, total cholesterol, triglycerides, LDL-cholesterol and vLDL-cholesterol levels and significantly increased the lowered serum insulin level, liver glycogen content, serum HDL-cholesterol level, homeostasis model assessment-insulin resistance (HOMA-IS) and HOMA-β cell function. The elevated cardiovascular risk indices in diabetic rats were significantly improved due to treatment with M. paradisiaca extracts. Concomitant with the increase in liver glycogen content, the glucose-6-phosphatase activity significantly decreased reflecting the decrease in hepatic glucose output. The heart function was potentially ameliorated as manifested by decrease in the elevated serum creatine kinase-MB, lactate dehydrogenase and aspartate aminotransferase activities after treatments of diabetic rats with M. paradisiaca extracts. The elevated liver lipid peroxidation and the decline in liver glutathione content and superoxide dismutase, glutathione peroxidase and glutathione-S-transferase activities were significantly reversed by treatments. Thus, it can be concluded that M. paradisiaca leaf and fruit peel hydroethanolic extracts may have antihyperlipidemic and cardioprotective potentials in NA/STZ-induced diabetic rats. These effects may be mediated via improvements in the glycemic state, β-cell function, tissue insulin sensitivity, and antioxidant defense mechanism.
本研究旨在评估芭蕉叶和果皮水醇提取物在烟酰胺（NA）/链脲佐菌素（STZ）诱导的糖尿病大鼠中的降血脂、心脏和抗氧化作用以及作用机制。实验性糖尿病通过单次腹腔注射 STZ（60mg/kg 体重），15 分钟后腹腔注射 NA（120mg/kg 体重）诱导。NA/STZ 诱导的糖尿病大鼠每天口服补充芭蕉叶和果皮水醇提取物，剂量为 100mg/kg 体重，共 28 天。用 M. paradisiaca 叶和果皮提取物治疗 NA/STZ 诱导的糖尿病大鼠，可显著降低空腹和餐后血清葡萄糖、总胆固醇、甘油三酯、LDL-胆固醇和 vLDL-胆固醇水平，并显著升高降低的血清胰岛素水平、肝糖原含量、血清高密度脂蛋白胆固醇水平、稳态模型评估-胰岛素抵抗（HOMA-IS）和 HOMA-β细胞功能。用 M. paradisiaca 提取物治疗可显著改善糖尿病大鼠升高的心血管风险指数。同时，肝糖原含量增加，葡萄糖-6-磷酸酶活性显著降低，反映肝葡萄糖输出减少。用 M. paradisiaca 提取物治疗糖尿病大鼠后，血清肌酸激酶-MB、乳酸脱氢酶和天冬氨酸转氨酶活性升高，心脏功能得到潜在改善。肝脂质过氧化升高，肝谷胱甘肽含量和超氧化物歧化酶、谷胱甘肽过氧化物酶和谷胱甘肽-S-转移酶活性下降，经治疗后均得到显著逆转。因此，可以得出结论，M. paradisiaca 叶和果皮水醇提取物可能具有抗高脂血症和心脏保护作用，可改善 NA/STZ 诱导的糖尿病大鼠的血糖状态、β细胞功能、组织胰岛素敏感性和抗氧化防御机制。
