Cho WonKyung, Mittal Sharad K, Elbasiony Elsayed, Chauhan Sunil K
Schepens Eye Research Institute of Mass Eye and Ear, Harvard Medical School, Boston, Massachusetts.
Schepens Eye Research Institute of Mass Eye and Ear, Harvard Medical School, Boston, Massachusetts.
Am J Pathol. 2021 Jun;191(6):1108-1117. doi: 10.1016/j.ajpath.2021.02.016. Epub 2021 Mar 8.
Mast cells, historically known for their function as effector cells in the induction of allergic diseases, reside in all vascularized tissues of the body, particularly, in proximity to blood and lymphatic vessels. Despite being neighboring sentinel cells to blood vessels, whether the spatial distribution of mast cells regulates the degree of angiogenesis remains to be investigated. Herein, an asymmetrical distribution of mast cells was shown at the murine ocular surface, with the higher number of mast cells distributed along the nasal limbus of the cornea compared with the temporal side. Using a well-characterized murine model of suture-induced corneal neovascularization, insult to the nasal side was shown to result in more extensive angiogenesis compared with that to the temporal side. To directly assess the impact of the spatial distribution of mast cell on angiogenesis, neovascularization was induced in mast cell-deficient mice (cKit). Unlike the wild-type (C57BL/6) mice, cKit mice did not show disproportionate growth of corneal blood vessels following the temporal and nasal insult. Moreover, cromolyn-mediated pharmacologic blockade of mast cells at the ocular surface attenuated the asymmetrical nasal and temporal neovascularization, suggesting that spatial distribution of mast cells significantly contributes to angiogenic response at the ocular surface.
肥大细胞，历史上因其在过敏性疾病诱导中作为效应细胞的功能而闻名，存在于身体所有有血管的组织中，特别是在血管和淋巴管附近。尽管肥大细胞是血管的邻近哨兵细胞，但肥大细胞的空间分布是否调节血管生成程度仍有待研究。在此，在小鼠眼表面显示出肥大细胞的不对称分布，与颞侧相比，沿角膜鼻侧分布的肥大细胞数量更多。使用特征明确的缝线诱导角膜新生血管形成的小鼠模型，与颞侧损伤相比，鼻侧损伤显示出更广泛的血管生成。为了直接评估肥大细胞空间分布对血管生成的影响，在肥大细胞缺陷小鼠（cKit）中诱导新生血管形成。与野生型（C57BL/6）小鼠不同，cKit小鼠在颞侧和鼻侧损伤后未显示角膜血管的不成比例生长。此外，色甘酸钠介导的眼表面肥大细胞药理学阻断减弱了不对称的鼻侧和颞侧新生血管形成，表明肥大细胞的空间分布显著促进眼表面的血管生成反应。
