Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China.
Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
J Pharm Biomed Anal. 2021 May 10;198:113999. doi: 10.1016/j.jpba.2021.113999. Epub 2021 Mar 2.
Ginsenoside Rg1 (Rg1) was one of the dominent active components in several Panax medicinal species as Panax notoginseng and Panaxginseng with diversified bioactivities. However, the study on tissue distribution of Rg1 remained limited and needed to be further explored for elucidation of its spatial distribution. In the present study, a LC-MS/MS combined with nanospray desorption electrospray ionization (DESI) mass spectrometry method was developed for exploration of tissue distribution of Rg1 at different time points after intravenous administration to rats. Furthermore, a MS inlet-heat method was developed to improve the imaging efficacy of Rg1 in brain tissue. The results obtained from LC-MS/MS analysis indicated that kidney possessed the highest tissue concentration, followed by liver, lung, spleen, heart and brain. Meanwhile, the elimination of Rg1 was swift within 1 h. For the spatial distribution of Rg1 by DESI-MS, Rg1 mainly accumulated in the pelvis section of kidney. Meanwhile, the imaging result of brain implied that Rg1 might be distributed in the pons and medulla oblongata region of brain at 15 min after intravenous administration. It is anticipated that the data on tissue distribution of Rg1 could provide references for further probing its efficacy and drug development.
人参皂苷 Rg1（Rg1）是几种人参药用植物中含量较高的活性成分之一，如三七和人参，具有多样化的生物活性。然而，Rg1 的组织分布研究仍然有限，需要进一步探索，以阐明其空间分布。在本研究中，建立了一种 LC-MS/MS 结合纳喷雾解吸电喷雾电离（DESI）质谱法，用于探索静脉注射大鼠后不同时间点 Rg1 的组织分布。此外，还开发了一种 MS 进样加热方法，以提高 Rg1 在脑组织中的成像效果。LC-MS/MS 分析结果表明，肾脏的组织浓度最高，其次是肝脏、肺、脾脏、心脏和大脑。同时，Rg1 在 1 小时内迅速消除。通过 DESI-MS 进行 Rg1 的空间分布研究，Rg1 主要积聚在肾脏的骨盆部分。此外，脑的成像结果表明，静脉注射后 15 分钟，Rg1 可能分布在脑桥和延髓区域。预计 Rg1 的组织分布数据将为进一步探索其功效和药物开发提供参考。
