Department of Botany, Gauhati University, Jalukbari, Guwahati, Assam, 781014, India.
Centre for Biotechnology, Maharshi Dayanand University, Rohtak, Haryana, 124 001, India.
Plant Physiol Biochem. 2021 May;162:221-236. doi: 10.1016/j.plaphy.2021.02.031. Epub 2021 Feb 26.
Long non-coding RNAs (lncRNAs) are a type of non-coding transcripts having length of more than 200 nucleotides lacking protein-coding ability. In the present study, 12807 lncRNAs were identified in Capsicum annuum tissues exposed to abiotic stress conditions viz. heat, cold, osmotic and salinity stress. Expression analysis of lncRNAs in different treatment conditions demonstrates their stress-specific expression. Thirty lncRNAs were found to act as precursors for 10 microRNAs (miRNAs) of C. annuum. Additionally, a total of 1807 lncRNAs were found to interact with 194 miRNAs which targeted 621 mRNAs of C. annuum. Among these, 344 lncRNAs were found to act as target mimics for 621 genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that out of those 621 gene sequences, 546 were tagged with GO terms, 105 Enzyme Code (EC) numbers were assigned to 246 genes and 223 genes are found to be involved in 63 biological pathways. In this report, we have highlighted the prospective role of lncRNAs in different abiotic stress conditions by interacting with miRNAs and regulating stress responsive transcription factors (TFs) such as DoF, WRKY, MYB, bZIP and ERF in C. annuum.
长链非编码 RNA（lncRNA）是一种长度超过 200 个核苷酸的非编码转录本，缺乏编码蛋白质的能力。在本研究中，鉴定了辣椒组织在非生物胁迫条件下（如热、冷、渗透和盐胁迫）的 12807 个 lncRNA。在不同处理条件下对 lncRNA 的表达分析表明，它们具有应激特异性表达。发现 30 个 lncRNA 可作为 10 个 miRNA（miRNA）的前体。此外，总共发现 1807 个 lncRNA 与 194 个靶向辣椒的 621 个 mRNA 的 miRNA 相互作用。其中，344 个 lncRNA 被发现作为 621 个基因的靶标模拟物。基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析表明，在这 621 个基因序列中，有 546 个基因被标记为 GO 术语，246 个基因被分配了 105 个酶代码（EC）编号，223 个基因参与了 63 个生物学途径。在本报告中，我们强调了 lncRNA 通过与 miRNA 相互作用，并调节 DoF、WRKY、MYB、bZIP 和 ERF 等应激响应转录因子，在不同非生物胁迫条件下的潜在作用。
