Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel.
Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel.
Methods Cell Biol. 2021;162:1-11. doi: 10.1016/bs.mcb.2020.08.001. Epub 2020 Sep 10.
Correlative light and electron microscopy (CLEM) combines the strengths of light microscopy (LM) and electron microscopy (EM) to pin-point and visualize cellular or macromolecular structures. However, there are many different imaging modalities that can be combined in a CLEM workflow, creating a vast number of combinations that can overwhelm new-comers to the field. Here, we offer a conceptual framework to help guide the decision-making process for choosing the CLEM workflow that can best address your research question, based on the answer to five questions.
相关光镜和电子显微镜技术（CLEM）结合了明场显微镜（LM）和电子显微镜（EM）的优势，可精确定位和可视化细胞或大分子结构。然而，在 CLEM 工作流程中可以结合许多不同的成像模式，从而产生大量的组合，可能会让该领域的新手感到不知所措。在这里，我们提供了一个概念框架，可根据五个问题的答案，帮助指导选择最能解决您的研究问题的 CLEM 工作流程的决策过程。
