Laerum O D, Sletvold O, Riise T
Gade Institute, Department of Pathology, University of Bergen, Norway.
Chronobiol Int. 1988;5(1):19-35. doi: 10.3109/07420528809078549.
The cell cycle distribution of bone marrow cells from the femurs of female C3H mice has been investigated by flow cytometry according to the time of the day and month of the year. Both circadian and seasonal variations were found for the different cell cycle phases as well as the total cell numbers per femur. Both the mesor, the acrophase and the amplitude of the S, G2 and (G1 + G0) phases varied significantly in some months, while in other months only insignificant rhythms were found. The relative cell cycle distribution only partly reflected variations in the total numbers of proliferating cells, since the total cell number per femur was also variable. The total numbers of cells in DNA synthesis seem to be higher in the first part of the year, indicating increased cell proliferation during winter and spring. In this period the acrophases of DNA synthesis and G2 were in the morning, while the second half of the year showed the peak later in the day. In general, hemopoietic cell proliferation seems to constitute a labile equilibrium with rapidly changing activities.
通过流式细胞术，根据一年中的月份和一天中的时间，对雌性C3H小鼠股骨骨髓细胞的细胞周期分布进行了研究。发现不同细胞周期阶段以及每根股骨的细胞总数均存在昼夜节律和季节性变化。在某些月份，S期、G2期和（G1 + G0）期的中值、高峰期和振幅均有显著变化，而在其他月份仅发现不显著的节律。相对细胞周期分布仅部分反映了增殖细胞总数的变化，因为每根股骨的细胞总数也是可变的。DNA合成中的细胞总数在一年的上半年似乎较高，表明冬春季节细胞增殖增加。在此期间，DNA合成和G2期的高峰期在上午，而下半年则在当天晚些时候出现峰值。一般来说，造血细胞增殖似乎构成了一个活动快速变化的不稳定平衡。
