Sletvold O, Laerum O D
Gade Institute, Department of Pathology University of Bergen, Haukeland Hospital, Norway.
Exp Gerontol. 1988;23(1):43-58. doi: 10.1016/0531-5565(88)90019-8.
The effect of aging on the cell cycle distribution in bone marrow cells was measured by flow cytometry with special reference to the lability in bone marrow physiology. Female C3H mice were examined every 3 h during a 24-h period at the age of 16, 21 and 26 months, vs 3-month-old control mice. Considerable circadian fluctuations were found in the different cell cycle phases in young mice. The rhythmicity patterns were different when comparing different months. In aging mice the variations were dampened, while consistent age-related phase shifts were not seen. The maximal 24-h mean numbers of cells in all three cell phases, but especially the S and G2 phases were reached at 21 months. The relative number of S and G2 phases were significantly reduced in 26-month-old mice, indicating an age-related shift of the proliferative capacity. The present findings are discussed in relation to age-related changes in granulopoiesis and the increase of myelotoxic effects during cancer chemotherapy in aging individuals.
通过流式细胞术测量衰老对骨髓细胞周期分布的影响，并特别参考骨髓生理学的不稳定性。在16、21和26月龄时，对雌性C3H小鼠在24小时内每3小时进行一次检查，并与3月龄对照小鼠进行比较。在年轻小鼠的不同细胞周期阶段发现了相当大的昼夜波动。比较不同月份时，节律模式有所不同。在衰老小鼠中，这种变化减弱了，而未观察到与年龄相关的一致的相位偏移。在21月龄时，所有三个细胞期的24小时平均细胞数达到最大值，尤其是S期和G2期。26月龄小鼠的S期和G2期相对数量显著减少，表明增殖能力存在与年龄相关的转变。结合衰老个体中粒细胞生成的年龄相关变化以及癌症化疗期间骨髓毒性作用的增加对本研究结果进行了讨论。
