Laboratório de Patologia Comparada, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
Departamento de Física, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
Sci Rep. 2021 Mar 11;11(1):5668. doi: 10.1038/s41598-021-85104-w.
Precise diagnosis and prognosis are key in prevention and reduction of morbidity and mortality in all types of cancers. Here we show that changes in the collagen fibres in the main histological subtypes of canine mammary gland carcinomas are directly associated with the tumour behaviour and the animal survival time and could become a useful tool in helping with diagnosis. Imaging by second harmonic generation and multiphoton excited fluorescence microscopy were performed to evaluate the collagen and cellular segment parameters in cancer biopsies. We present a retrospective study of 45 cases of canine mammary cancer analysing 836 biopsies regions including normal mammary gland tissue, benign mixed tumours, carcinoma in mixed tumour, carcinosarcoma, micropapillary carcinoma and solid carcinoma. The image analyses and the comparison between the tumour types allowed to assess the collagen fibre changes during tumour progression. We demonstrate that the collagen parameters correlate with the clinical and pathological data, the results show that in neoplastic tissues, the collagen fibres are more aligned and shorter as compared to the normal tissues. There is a clear association of the mean fibre length with the dogs survival times, the carcinomas presenting shorter collagen fibres indicate a worse survival rate.
精确的诊断和预后是预防和降低各类癌症发病率和死亡率的关键。在这里，我们表明，犬乳腺肿瘤主要组织学亚型中胶原纤维的变化与肿瘤的行为和动物的生存时间直接相关，并可能成为帮助诊断的有用工具。通过二次谐波产生和多光子激发荧光显微镜进行成像，以评估癌症活检中的胶原和细胞段参数。我们回顾性研究了 45 例犬乳腺肿瘤，分析了包括正常乳腺组织、良性混合瘤、混合瘤中的癌、癌肉瘤、微乳头状癌和实体癌在内的 836 个活检区域。图像分析和肿瘤类型之间的比较允许评估肿瘤进展过程中胶原纤维的变化。我们证明胶原参数与临床和病理数据相关，结果表明在肿瘤组织中，胶原纤维比正常组织更规则且更短。纤维长度的平均值与狗的生存时间有明显的关联，胶原纤维较短的癌表明生存率更差。
